吴志平,赵晓昆,吕晨
WU Zhiping1,ZHAO Xiaokun2,LV Chen2(1.Department of Urology

• 1:贵阳医学院附院泌尿外科
• 2:中南大学湘雅二医院泌尿外科

摘要(Abstract)：

目的:探讨β-胡萝卜素对人膀胱癌T24细胞细胞增殖和凋亡的影响。方法:不同浓度的β-胡萝卜素作用于T24细胞,MTT法检测细胞增殖,流式细胞术检测T24细胞的凋亡指数。结果:MTT法检测结果显示10μmol/L和20μmol/L组β-胡萝卜素均能明显抑制T24细胞的生长,抑制率分别为28.83%和63.02%(P<0.05);流式细胞术检测显示β-胡萝卜素能诱导T24细胞调亡,5μmol/L组T24细胞的凋亡指数为(0.065±0.018)(P<0.05),10μmol/L和20μmol/L组凋亡指数分别为(0.126±0.022)和(0.190±0.024)(P<0.01),呈现剂量依赖效应,10μmol/L组及20μmol/L组Hochest染色可见凋亡小体形成。结论:β-胡萝卜素抑制T24细胞的生长,并且呈现剂量依赖效应,其机制可能为诱导细胞凋亡。
Objective: To explore the effects of β-carotene on proliferation and apoptosis of human bladder cancer cell line T24.Methods: T24 cells were treated with different concentrations of β-carotene for 72 hours.Proliferation of T24 cells was tested with Hochest staining and MTT assay.T24 cell apoptosis was determined with flow cytometry.Results: MTT assay showed that β-carotene at concentrations of 10 μmol/L and 20 μmol/L could significantly inhibit T24 cell proliferation,and the inhibition rates were 28.83% and 63.02% respectively(P<0.05).Flow cytometry showed that β-carotene could induce T24 cell apoptosis,apoptotic index(AI) of 5 μmol/L group was 0.065±0.018(P<0.05),with the increase of β-carotene concentration,AI increased,AI of 10 μmol/L and 20 μmol/L groups were 0.126±0.022 and 0.190±0.024 respectively(P≤0.01),showing a dose-dependent manner.Apoptotic bodies were detectable when the cells stained by Hochest method.Conclusions: β-carotene could inhibit T24 cell proliferation in a dose-dependent manner,the mechanism may be associated with inducing T24 cell apoptosis.

关键词(KeyWords)： β-胡萝卜素;癌;膀胱;肿瘤细胞,培养的;细胞凋亡;流式细胞术
beta-carotene;carcinoma;bladder;tumor cells,culture;apoptosis;flow cytometry

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 吴志平,赵晓昆,吕晨
WU Zhiping1,ZHAO Xiaokun2,LV Chen2(1.Department of Urology

DOI: 10.19367/j.cnki.1000-2707.2012.02.013

参考文献(References)：

• [1]Bouayed J,Bohn T.Exogenous antioxidants-double-edgedswords in cellular redox state:health beneficial effects atphysiologic doses versus deleterious effects at high doses[J].Oxid Med Cell Longev,2010(4):228-237.
• [2]Lin FM,Chen LR,Lin EH,et al.Compounds fromwedelia chinensis synergistically suppress androgen activi-ty and growth in prostate cancer cells[J].Carcinogene-sis,2007(12):2521-2529.
• [3]Jain M,Miller AB,To T.Premorbid diet and the progno-sis of women with breast cancer[J].J Natl Cancer Inst,1994(18):1390-1397.
• [4]Lodi G,Sardella A,Bez C,et a1.Interventions for trea-ting oral leukoplakia[J].Cochrane Database Syst Rev,2006(4):1829.
• [5]Roswall N,Olsen A,Christensen J,et al.Micronutrient in-take and risk of urothelial carcinoma in a prospective Dan-ish cohort[J].Eur Urol,2009(5):764-770.
• [6]张业楼,张月明,刘命宝,等.β-胡萝卜素与番茄红素体外抗肿瘤作用研究[J].新疆医科大学学报,2002(1):32-34.
• [7]Palozza P,Calviello G,Serini S,et al.β-Carotene athigh concentrations induces apoptosis by enhancing oxy-radical p roduction in human adenocarcinoma cells[J].Free Radic Biol Med,2001(9):1000-1007.
• [8]张军,张敬,赵燕,等.β-胡萝卜素对白血病HL-60细胞凋亡及细胞周期分布的影响[J].营养学报,1999(4):401-404.
• [9]张敬,张军,石红军,等.β-胡萝卜素诱导白血病细胞凋亡[J].上海铁道大学学报,1998(9):12-14.
• [10]Chan LL,Lai N,Wang E,et al.A rapid detection meth-od for apoptosis and necrosis measurement using the Cel-lometer imaging cytometry[J].Apoptosis,2011(12):1295-303.

文章评论(Comment)：