兰咏哲,李启瑞,黄劲,赵亮,董宇华,阚雨,王迪,廖万清,康颖倩
LAN Yongzhe,LI Qirui,HUANG Jin,ZHAO Liang,DONG Yuhua,KAN Yu,WANG Di,LIAO Wanqing,KANG Yingqian

• 1:贵州医科大学基础医学院微生物学教研室
• 2:贵州省微生物与人类健康关系研究人才基地&贵州省普通高校病原生物学特色重点实验室
• 3:贵州医科大学
• 4:贵州医科大学基础医学院生物化学与分子生物学教研室

摘要(Abstract)：

目的:改良姜黄药渣真菌分离方法,探究姜黄药渣真菌的多样性。方法:采用五点法采集姜黄药渣样本,分别采用常规真菌分离方法和改良方法分离样本中的真菌,通过聚合酶链式反应(PCR)扩增其ITS基因序列后测序;序列结果在美国国立生物技术信息中心(NCBI)数据库进行下载与比对,确定菌株种属并计算分离率。结果:采用常规真菌分离方法和改良方法分别从姜黄药渣样本中分离获得菌株13株和222株,前者分离菌株均数低于后者,差异有统计学意义(P <0. 05);采用常规真菌分离方法和改良方法分离获得菌株分别属于2个门5个属的6个菌种和3个门12个属的20个菌种,且青霉属均为优势菌属(分离率分别为61. 53%、46. 84%),Aspergillus minisclerotigenes分离出的菌株数均最多、为优势菌种。结论:姜黄药渣中真菌多样性丰富,且改良方法相较于常规真菌分离方法分离的菌株数量增加,且不会改变姜黄药渣中真菌菌株的优势菌属及菌种。
Objective: To improve the separation method of turmeric residue fungi,and to analyze its diversity. Methods: Five turmeric residue samples were collected by the five-point method. The fungi were separated by the conventional method and the improved method respectively. And internal transcribed spacer( ITS) gene sequence was amplified by polymerase chain reaction( PCR) and sequenced. The fragments of ITS were compared through the NCBI BLAST( https://blast. ncbi. nlm.nih. gov) to identify the taxonomy status of isolates and calculate the separation rate. Results: 13 and222 strains were isolated from 5 samples of turmeric residue by the conventional method and the improved method,respectively. The mean number of strains isolated by the former method was lower than that of the improved method,and the difference was statistically significant( P < 0. 05). The strains were isolated and obtained by the conventional method and the improved method,belonging to 6 species in 5 genera of 2 phylum and 20 species in 12 genera of 3 phylum,respectively,and Aspergillus was the dominant genus( the separation rates were 61. 53% and 46. 84%,respectively).And Aspergillus minisclerotigenes were dominant strains. Conclusion: The fungi of turmeric residue are characterized by diversity. Compared with the conventional method,the number of strains isolated by the improved method increases,and the dominant genera and strains in the turmeric residue will not be changed.

关键词(KeyWords)： 真菌;姜黄药渣;多样性;分离方法;技术改良;木质纤维素
fungi;turmeric residue;diversity;separation method;technical improvement;lignocellulosic

Abstract:

Keywords:

基金项目(Foundation): 国家科技部及教育部“111计划”[国科发专(2019)475];; 贵州省第六批人才基地项目[RCJD2018-22];; 贵州省科技厅社会发展攻关支撑项目[黔科合支撑(2019)2873];贵州省科技厅优秀青年科技人才培养计划项目[黔科合(20175639)];; 贵州省科学技术厅国际科技合作基地[黔科合平台人才(2020)4101]

作者(Author): 兰咏哲,李启瑞,黄劲,赵亮,董宇华,阚雨,王迪,廖万清,康颖倩
LAN Yongzhe,LI Qirui,HUANG Jin,ZHAO Liang,DONG Yuhua,KAN Yu,WANG Di,LIAO Wanqing,KANG Yingqian

DOI: 10.19367/j.cnki.2096-8388.2020.09.003

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