曾柱,杨海岳,张平,谢利德,肖俊
ZENG Zhu 1; YANG Hai yue 2; ZHANG Ping 3; XIE Li de 4; XIAO Jun 1 (1.Department of Medical Physics;

• 1:贵阳医学院物理教研室!贵州贵阳550004
• 2:承德高等师范专科学校!河北承德067000
• 3:承德医学院物理教研室!河北承德067000
• 4:北京大学基础医学院血液流变研究中心!北京100083

摘要(Abstract)：

目的 :研究新生在体红细胞变形和取向的变化。方法 :用苯肼使动物造成急性溶血性贫血的方法 ,建立了一种由急性溶血性贫血后而诱发动物幼细胞增多的非正常生理状态的红细胞衰老模型 ,继而用新型激光衍射法研究新生红细胞在产生到死亡过程中变形和取向能力的改变。结果 :对新生红细胞的压积、变形指数、取向指数等指标的连续 6 0多天的监测 ,发现红细胞在整个衰老过程中的变形指数和取向指数有明显改变。结论 :新生红细胞的变形和取向能力比衰老红细胞的变形和取向能力要强
Objective:To study the deformation and orientation of RBCs under abnormal physiological conditions of phenylhydrazine injection in vivo. Mothods: A models of anemia in rabbits induced by injection of phenylhydrazine was built. To study the deformation and orientation of RBCs with New Ektacytometry. Results: The measurements of hemacrit, deformation index and orientation index etc were performed in the processes of RBC senescence in vivo for more than 60 days. Conclusion: It was shown that there was obvious changes in the RBC's deformation and orientation in the senescent models. The ability to deformation and orientation is better in younger RBCs than in those olders.

关键词(KeyWords)： 苯肼类;红细胞变形性;红细胞指数;红细胞比容;取向指数;新型激光衍射法
phenylhydrazines;erythrocyte deformability; erythrocyte indixes;hematocrit;orientation indexes;new ektacytometry

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目!(39770 2 0 3)

作者(Authors): 曾柱,杨海岳,张平,谢利德,肖俊
ZENG Zhu 1; YANG Hai yue 2; ZHANG Ping 3; XIE Li de 4; XIAO Jun 1 (1.Department of Medical Physics;

DOI: 10.19367/j.cnki.1000-2707.2001.01.001

参考文献(References)：

• [1]BrugnaraC ,DeFL .Effectofageandphenylhydrazineonthecationtransportpropertiesofrabbiterythrocytes[J].JCellPhysiol,1993 ,15 4:2 71- 2 80
• [2 ]SuzukiT ,DaleGL .Biotnylatederythrocytes:invivosur vivalandinvitrorecovery [J].1987,70 :791- 76 6
• [3]WanghRE .Reductionoferythrocytemembranevis coelasticcoefficientsreflectspectrindeficienciesinhered itaryspherocytosis[J].JClinInvest,1988,81:133 -141.
• [4 ]AnneM ,Mortensen ,RaymondF .Novakenhancedpro teolysisandchangesinmembraneassociatedcalpainfol lowingphenylhydrazineinsulttohumanredcells[J].ToxicologyandAppliedPharmacology ,1991,110 ,435 -44 9
• [5 ]孙大公 ,施岩 ,严宗毅 ,等 胰蛋白酶对红细胞膜生物物理特性的影响 [J] 生物物理学报 ,1997,13(4 ) :5 74- 8
• [6 ]FischerT ,HSchmid -Schonbein .Tanktreadmotionofredcellmembraneinviscometicflow :behaviorofintracellularmarkers (withfilm ) [J].BloodCells,1971,3:35 1- 36 5
• [7]KholeifIA .MotionofasingleRBCinplansheraflow[J].Biorheology ,1974,11:337- 348.
• [8]MBitbol.Redbloodcellorientationinorbitc =0[J].BiophysicalJournal,1986 ,49:10 5 5 - 10 6 8
• [9]文宗曜 ,马维原 ,李田勋 ,等 .红细胞膜结构与其微观流变性的关系研究 [J] 中国生物医学工程学报 ,1995 ,14(1) :2 8- 32

文章评论(Comment)：