张红玲,杨明,戴佳琳,黄映康,黄江,王杰
ZHANG Hongling1,YANG Ming2,DAI Jialin1,HUANG Yingkang1,HUANG Jiang1,WANG Jie1(1.Department of Forensic Medicine

• 1:贵阳医学院法医学教研室
• 2:贵阳医学院生物学教研室

摘要(Abstract)：

目的:构建贵阳地区常见嗜尸性蝇类丽蝇科的系统发育树。方法:对家兔尸体上采集到的嗜尸性蝇类丽蝇科的卵、幼虫及成虫提取DNA,扩增COI基因条形码区的序列,采用CLC Sequence View、MEGA软件进行序列分析,构建系统发育树。结果:共采集到丽蝇科5属9种嗜尸性蝇类,经序列测定确定各样本的形态学鉴定与分子鉴定是符合的,并以此序列构建出贵阳地区常见嗜尸性蝇类丽蝇科的系统发育树。结论:可以利用COⅠ基因条形码区序列构建的系统发育树进行贵阳地区嗜尸性蝇类的种属鉴定。
Objective: To establish the phylogenetic tree of common calliphoridae sarcosaprophagous fly in Guiyang city,and so as to provide basis for identification of eggs and larvae of calliphoridae sarcosaprophagous fly in Guiyang city.Methods: DNA of eggs,larvae and adults of sarcosaprophagous fly(calliphoridae) was extracted from rabbit corpse.Sequence of barcode region of cytochrome oxidase I(COI) gene was amplified by PCR.Softwares such as CLC sequence view and MEGA were employed to analyze the sequence.The phylogenetic tree of sarcosaprophagous fly was established.Results: A total of 5 genus and 9 species calliphoridae sarcosaprophagous fly were collected.After identifying the sequences and removing primers and same sequences,the phylogenetic tree of calliphoridae sarcosaprophagous fly in Guiyang city was established.Conclusions: The phylogenetic tree of calliphoridae sarcosaprophagous fly in Guiyang city is established based on sequence of barcode region of COI gene,which provides the basis for identification of calliphoridae sarcosaprophagous fly in Guiyang.

关键词(KeyWords)： 法医学;昆虫学;蝇科;序列分析,DNA
forensic medicine;entomology;muscidae;sequence analysis,DNA

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技攻关项目[黔科合NY字(2010)3090]

作者(Author): 张红玲,杨明,戴佳琳,黄映康,黄江,王杰
ZHANG Hongling1,YANG Ming2,DAI Jialin1,HUANG Yingkang1,HUANG Jiang1,WANG Jie1(1.Department of Forensic Medicine

DOI: 10.19367/j.cnki.1000-2707.2012.05.010

参考文献(References)：

• [1]胡萃.法医昆虫学[M].重庆:重庆出版社,2000:1-23.
• [2]赵子琴.法医病理学[M].北京.人民卫生出版社,2010:84-87.
• [3]Smith KGV.A manual of forensic entomology[M].Lon-don:British Museum(Natural History),1986:40-63.
• [4]Sperling FAH,Anderson GS,Hickey DA.A DNA-basedapproach to the identification of insect species used forpostmortem interval estimation[J].J Forensic Sci,1994(2):418-427.
• [5]Seong HP,Zhangyong,Huguo Piao,et al.Use of cyto-chrome c oxidase subunit I(COI)nucleotide sequencesfor identification of the Korean Luciliinae Fly species(Diptera:Calliphoridae)in forensic investigations[J].JKorean Med Sci,2009(24):1058-1063.
• [6]胡佳林,郑学礼,陈晓光.中国7市家蝇rDNA-ITS2基因分析[J].第四军医大学学报,2008(11):961-963.
• [7]Hebert PDN,Cywinska A,Ball SL,et a1.Biological identi-fications through DNA barcodes[J].Proc R Soc London(Series B),2003(270):313-321.
• [8]程佳月,王丽华,彭克美,等.国际生命条形码计划———DNA Barcoding[J].中国畜牧兽医,2009(8):49-53.
• [9]刘勇,宋毓,李晓宇.基于线粒体COⅠ基因的DNA条形码技术在昆虫分子鉴定中的应用[J].植物检疫,2010(2):46-50.
• [10]武宇鹏,丁亮,李捷,等.DNA条形码的应用进展及讨论[J].环境昆虫学报,2011(1):99-106.
• [11]马英,鲁亮.DNA条形码技术研究新进展[J].中国媒介生物学及控制杂志,2010(3):275-280.
• [12]陈禄仕.贵州省尸食性蝇类的种类和分布[J].昆虫学报,2004(6):849-853.
• [13]范滋德.中国常见蝇类检索表[M].2版.北京:科学出版社,1992:465.
• [14]薛万琦,赵建铭.中国蝇类[M].沈阳:辽宁科学出版社,1996:1438-1447.

文章评论(Comment)：