莫翼军,黄韵祝,黄健
MO Yijun,HUANG Yunzhu,HUANG Jian (Department of Clinic Biochemistry

• 1:贵阳医学院临床生物化学教研室

摘要(Abstract)：

目的:探讨载脂蛋白M(apo M)基因启动子区多态性在贵州省汉族、苗族及布依族人群之间的差异,并分析单核苷酸多态性对apo M水平及血脂水平的影响。方法:奥林巴斯AU5400全自动生化分析仪测定血清甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C),应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)对150例汉族、140例苗族及140例布依族人群血标本apo M启动子区域T-778C进行基因多态性分析,应用酶联免疫吸附试验(ELISA)试剂盒测定血清apo M。结果:汉族人群apoM-778位点C等位基因频率明显高于苗族人群(P=0.005)与布依族人群(P=0.005),苗族与布依族人群apoM-778位点C等位基因频率无差异,apo M-778位点CT+CC型血清apo M及HDL-C含量均低于TT型(P=0.026,P=0.048)。结论:apo M基因启动子区T-778C存在民族差异,汉族人群apo M-778位点C等位基因遗传携带率高于苗族与布依族,同时apo M-778位点C等位基因引起血清apo M水平和HDL-C水平降低。
Objective: To investigate whether there are differences in the promoter region of apolipoprotein M(apo M) gene among different nationalities,and to explore the effect of mononucleotide polymorphism on apo M levels and serum lipid levels.Methods: Triglyceride(TG),cholesterol(TC),high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C) were determined with Olympus AU5400 automatic biochemistry analyzer.Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) was employed to analyze polymorphism of apo M gene promoter region T-778C in blood preparations of 150 Han,140 Miao,and 140 Buyi nationality healthy subjects in Guizhou province.Enzyme linked immunosorbent assay was used to measure the serum apo M levels.Results: The frequency of C allele of apo M-778 site in Han nationality was higher than those in Miao(P=0.005) and Buyi(P=0.005) nationalities.No difference was found in the frequencies between Miao and Buyi nationality.Serum levels of apo M and HDL-C of which apo M-778 site was CT+CC genotype were lower than those of TT genotype(P=0.026,P=0.048).Conclusions: The ethnic differences exist in apo M gene promoter region T-778C.The frequency of C allele of apo M-778 site in Han nationality is higher than that in Miao and Buyi nationalities.C allele at apo M-778 site causes the decrease of serum apo M and HDL-C levels.

关键词(KeyWords)： 载脂蛋白类;多态性,单核苷酸;血脂;酶联免疫吸附测定;少数民族
apolipoproteins;polymorphism,single nucleotide;serum lipids;enzyme-linked immunosorbent assay;minority groups

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金(E2009-9)

作者(Author): 莫翼军,黄韵祝,黄健
MO Yijun,HUANG Yunzhu,HUANG Jian (Department of Clinic Biochemistry

DOI: 10.19367/j.cnki.1000-2707.2011.05.011

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