张敏;高秀丽;鲍鹏;
ZHANG Min,GAO Xiu-li,BAO Peng(School of Pharmacology,Guiyang Medical College,Guiyang 550004,China)

• 1:贵阳医学院药学系
• 2:贵阳医学院药学系
• 3:贵阳医学院药学系 贵州贵阳550004
• 4:贵州贵阳550004

摘要(Abstract)：

目的:探讨大豆和经过发酵后的豆豉游离氨基酸总量的变化。方法:采用氨基酸的游离氨基与水合茚三酮产生显色反应,在一定范围内,其颜色的深浅与氨基酸的含量成正比。结果:波长570nm处含氮量在0～5μg吸收度值与含氮量值线性关系良好,r=0.9999(n=5),且豆豉中游离氨基酸总量与大豆中游离氨基酸总量存在显著性差异。结论:大豆在经过发酵后游离氨基酸总量产生明显变化,更易被人体所吸收,其营养价值更高。
Objective:To test and compare the free amino acid contents and their changes in fermented and un-fermented soybeans for the assessment of the nutritional value of the former.Methods:Color reaction of free amino acids with ninhydrin was adopted,since,in certain extent,the color depth is correlated with the amino acid content.Results:A good linearity in the range of 0-5 μg(r= 0.999 9,n=5)was obtained.The total quantity of free amino acids in fermented soybeans was higher than that in unfermented soybeans.The difference was significant.Conclusion:Since free amino acids are easier absorbed by human body,and the total amount of free amino acids in soybean obviously increase after fermentation,it is concluded that fermented soybean has much higher nourishing value.

关键词(KeyWords)： 氨基酸类;黄豆;发酵;食品分析
amino acids;soybeans;fermentation;food analysis

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 张敏;高秀丽;鲍鹏;
ZHANG Min,GAO Xiu-li,BAO Peng(School of Pharmacology,Guiyang Medical College,Guiyang 550004,China)

DOI: 10.19367/j.cnki.1000-2707.2006.05.012

参考文献(References)：

• [1]杨艳燕,李顺意,高尚,等.豆豉中纳豆杆菌的筛选和纳豆激酶的初步分离[J].沈阳药科大学学报,2001,18(6):436-442.
• [2]杨晓斌,谢拥葵,韦燕鹏,等.益生菌纳豆芽孢杆菌的筛选[J].广州食品工业科技,2003,19(1):9-13.
• [3]王文平.植物样品中游离氨基酸总量测定方法的改进[J].北京农学院学报,1998,13(3):9-13.
• [4]李小红.大豆在我国膳食中的应用前景分析[J].作物研究,1997(1):37-39.
• [5]马毓霞,王勇,黄威,等.纳豆开发前景广阔[J].2004(11):37-38.

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