王林,王大寿,穆琼,姚旌,陆巍,沃春新
WANG Lin,WANG Dashou,MU Qiong,YAO Jin,LU Wei,WO Chunxin(Department of Pain Management

• 1:贵阳医学院附院疼痛科

摘要(Abstract)：

目的:探讨臭氧治疗对家兔骨性关节炎(OA)关节冲洗液中NO含量的影响。方法:40只新西兰兔随机分为5组,A组为正常对照组,B组为模型对照组,C、D、E组分别为臭氧(20、40、60 mg/L)治疗组;用Vand-m an法造模型成功后,将不同浓度的臭氧注射入C、D、E组兔膝关节腔内,每3天1次,连续2次;检测关节冲洗液中NO的含量,观察关节软骨的病理变化并进行病理评分。结果:B组软骨呈典型的OA改变,C、D组关节软骨可见不同程度修复,病理评分与B组相比有显著性差异(P<0.01或P<0.05);E组未见明显修复,病理评分与B组无显著差异;与A组相比,B组关节冲洗液中NO含量显著增高(P<0.01);与B组相比,C、D治疗组NO含量显著降低(P<0.01),E组NO显著增高(P<0.01)。结论:20~40 mg/L浓度的臭氧可促进实验性OA关节软骨修复,其机制可能与抑制NO生成有关,60 mg/L浓度的臭氧无软骨修复作用。
Objective: To explore the impact of ozone treatment to rabbit osteoarthritis(OA) and nitrogen monoxidum(NO) contents in joint flushing fluid(JFF).Methods: Forty New Zealand rabbits were randomly divided into five groups: normal control group(group A),model control group(group B),and treatment groups C,D,and E which were treated with ozone at doses of 20 mg/L,40 mg/L,and 60 mg/L respectively.Models were established with Vandman method successfully,and different doses of ozone were injected into rabbit knee joint cavity of groups C,D,and E twice a week.NO contents in JFF and articular cartilage variation were determined and pathological changes in arthrodial cartilage were observed and analyzed.Results: Typical OA changes in cartilage were found in group B,while different degrees of repairing on articular cartilage could be seen in groups C and D of which the pathological scores differed from that of group B significantly(P<0.01,P<0.05),No obvious repairing was found in group E,of which the pathological score did not differ from that of group B.NO content in JFF was significantly higher in group B than in group A(P<0.01);NO contents in JFF of groups C and D were obviously lower than that in group B(P<0.01),while in group E,NO content significantly increased(P<0.01).Conclusions: Ozone with concentrations of 20~40 mg/L can promote articular cartilage repair in experimental OA,and its mechanism may relate to inhibiting NO generation.Ozone of 60 mg/L has no repairing effect to cartilage.

关键词(KeyWords)： 骨关节炎;臭氧;软骨,关节;一氧化氮;兔
osteoarthritis;ozone;cartilage,articular;nitric oxide;rabbits

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金资助项目(黔科合J字[2007]2103)

作者(Author): 王林,王大寿,穆琼,姚旌,陆巍,沃春新
WANG Lin,WANG Dashou,MU Qiong,YAO Jin,LU Wei,WO Chunxin(Department of Pain Management

DOI: 10.19367/j.cnki.1000-2707.2010.04.007

参考文献(References)：

• [1]Ying KN,While A.Pain relief in osteoarthritis and rheu-matoid arthritis:TENS[J],Br J Community Nurs,2007(8):270-279.
• [2]任芹,赵序利,万燕杰.医用臭氧治疗膝骨性关节炎的疗效观察[J].实用疼痛学杂志,2006(2):67.
• [3]Videman T.Experimental osteoarthritis in the rabbit:comparision of different periods repeated immobilization[J].Acta Orthop Scard,1982(3):339-347.
• [4]毕胜,王福根,侯京山,等.细胞因子在髌下脂肪垫损伤动物模型中的变化[J].中国疼痛医学杂志,2000(2):86.
• [5]CB Little,CR Flannery,CE Hughes,et al.Cytokine in-duced metalloproteinase expression and activity does notcorrelate with focal susceptibility of articular cartilage todegeneration[J].Osteoarthritis and Cartilage,2005(2):162-170.
• [6]高石军,陈百成,汤慧华,等.一氧化氮在骨性关节炎发病机制中的作用[J].中华骨科杂志,1999(7)411.
• [7]Deschner J,hofman CR,Piesco NP,et,al.Signal trans-duotion by mechanical strain in chondrocytes[J].CurrOpin Clin Nutr Metab Care.2003(3):289-293.
• [8]Vuolteenabo K,Moilanen T,hamalainen M,et al.Regula-tion of nitric oxide production in osteoarthritis and rheuma-tid cartilage[J].Scand J rheumatol,2003(1):19-24.
• [9]Pelletier J.Reduction in the structural changes of experi-mental osteoarthritis by a nitric oxide inhitor[J].Osteo-arthritis Cartilage1999(7):416.
• [10]金大地,孙炜,王吉兴,等.一氧化氮合酶抑制剂对骨性关节炎的潜在治疗意义[J].中华骨科杂志,2002(6):367.

文章评论(Comment)：