吴昌学,曾小箐,单可人,李毅
WU Chang-xue; ZENG Xiao-qing; SHAN Ke-ren; LI Yi ;

• 1:贵阳医学院分子生物学重点实验室
• 2:贵阳医学院附院血液内科
• 3:贵阳医学院分子生物学重点实验室
• 4:贵阳医学院分子生物学重点实验室 贵州 贵阳 550004

摘要(Abstract)：

目的:研究慢性粒细胞性白血病患者bcr/abl融合基因阳性率及其临床意义。方法:取慢性粒细胞性白血病(CML)患者骨髓或外周血,提取单核细胞的总RNA,用逆转录多聚酶链反应(RT-PCR),观察其bcr/abl基因的表达。结果:9例CML患者中,8例bcr/abl基因阳性,占88.9％。结论:bcr/abl基因检测可以用来辅助CML的临床诊断。
Objective:To investigate the positive expression rate of bcr/abl fusion gene in chronic myelogenous leukemia (CML) and its clinical significance. Methods: The RT-PCR technique was used to detect the bcr/abl gene in CML. Results: Out of 9 cases of chronic phase of CML, 8 showed positive expression of bcr/abl gene which accounted for 88. 9% . Conclusions: Bcr/abl gene detecting technique has significance in clinical classification of CML. When bcr/abl gene expresses positively, it contributes to differential diagnosis in blast crisis of CML and acute myelogenous leukemia ( AML) at the first diagnosis.

关键词(KeyWords)： 白血病;髓样;慢性期;基因;abl;基因;bcr;逆转录聚合酶链反应
leukemia, myeloid, chronic; genes, abl; genes, bcr; reverse transcriptase polymerase chain reaction

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 吴昌学,曾小箐,单可人,李毅
WU Chang-xue; ZENG Xiao-qing; SHAN Ke-ren; LI Yi ;

DOI: 10.19367/j.cnki.1000-2707.2003.04.014

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