邹佳宁,宋聚先,常楚瑞,王晓丽
ZOU Jianing1,SONG Juxian1,CHANG Churui2,WANG Xiaoli2(1.Lab for Molecular Pharmacognosy

• 1:贵阳医学院分子生药学实验室
• 2:贵阳医学院分子生药学实验室
• 3:贵阳医学院药用植物与生药学教研室
• 4:贵阳医学院药用植物与生药学教研室 贵州贵阳550004

摘要(Abstract)：

目的:以天麻块茎为材料,研究不同的预处理方法对DNA提取的纯度和浓度的影响。方法:将新鲜天麻块茎切成薄片,分别置于生理盐水,灭菌双蒸水及不同浓度的乙醇溶液中,于4℃存放5~7 d,再分别用CTAB法和SDS法提取其总DNA。结果:样品采用浓度为30%~50%的乙醇溶液浸泡后再提取,获得的DNA浓度和纯度都较为理想,而经生理盐水和灭菌双蒸水预处理后提取的样品DNA虽纯度不理想,但浓度较高。结论:采用30%~50%的的乙醇溶液可作为天麻块茎总DNA提取前的预处理液,生理盐水和灭菌双蒸水适用于样品的长期保存。
Objective: Different pretreatment methods affecting purity and concentration of DNA extraction of Rhizoma Gastrodiae tuber were studied.Methods: Tubers of fresh samples were cut into thin pieces and placed in physiologic saline,sterilized ddH2O or ethanol of different concentrations respectively.After them being stored at 4 ℃ for 5-7 days,genomic DNA was extracted with CTAB and SDS methods.Result: Both purity and concentration of DNA extracted with pretreatment of 30%～50% ethanol were more ideal,while the DNA extracted with pretreatment of physiologic saline or sterilized ddH2O showed higher concentration but lower purity.Conclusion: 30%-50% etnanol pretreatment is better for DNA extraction of Rhizoma Gastrodiae tuber,while physiologic saline and sterilized ddH2O are suitable for long-term sample storing.

关键词(KeyWords)： 天麻;DNA;乙醇;氯化钠
Gastrodiae elata;DNA;ethanol;sodium chloride

Abstract:

Keywords:

基金项目(Foundation): 科技部2004BA721A33;; 贵州省科技黔科合农社字(2001)1125号,黔科合中药专字[2003]51号

作者(Author): 邹佳宁,宋聚先,常楚瑞,王晓丽
ZOU Jianing1,SONG Juxian1,CHANG Churui2,WANG Xiaoli2(1.Lab for Molecular Pharmacognosy

DOI: 10.19367/j.cnki.1000-2707.2007.02.005

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