梁光义,曹佩雪
LIANG Guang-yi; CAO Pei-xue (The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences ;

• 1:贵州省中国科学院天然产物化学重点实验室
• 2:贵州省中国科学院天然产物化学重点实验室 贵州 贵阳 550002
• 3:贵州 贵阳 550002

摘要(Abstract)：

目的:采用反相高效液相色谱法测定大黄药材及其单味中药精制颗粒中大黄酚的含量。方法:以Hypersil C_(18)柱为分析柱,以甲醇-0.04％磷酸(85:15)为流动相测定,检测波长为254nm。结果:大黄酚线性范围0.0151～1.5100μg。结论:本法简便、准确、重复性好,可用于大黄单味药定量分析。
Objective:To investigate the content of chrysophanol in a kind of refined granules. Methods: The content of chrysophanol was determined with RP - HPLC which was consisted of C18 column and methanol-0.04 % phosphoris acid (85; 15) mixture as mobile phase, and the detection wavelength was 254 nm. Results: The linear range of chrysophanol was 0.0151 - 1. 51μg. Conclusion: This method is simple and accurate, and has a good reproducibility and can be used as a method for quantitative analysis.

关键词(KeyWords)： 中草药;色谱法;高压液相;大黄酚
drugs, Chinese herbal; chromatography, high pressure liquid; chrysophanol

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(39960083)

作者(Authors): 梁光义,曹佩雪
LIANG Guang-yi; CAO Pei-xue (The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences ;

DOI: 10.19367/j.cnki.1000-2707.2003.04.006

参考文献(References)：

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• [2] 赵爱华,刘金德,王振中.HPLC法测定妇乐冲剂中大黄酸、大黄素、大黄酚的含量[J].基层中药杂志,1998,12(3) :36．
• [3] 国家药典委员会.中华人民共和国药典[S].北京:化学工业出版社,2000． 18．

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