张敏,王鹏娇,舒阳,孟小夏,王沁,月清仪,高秀丽
ZHANG Min,WANG Pengjiao,SHU Yang,MENG Xiaoxia,WANG Qin,YUE Qingyi,GAO Xiuli (School of Pharmacology

• 1:贵阳医学院药学院

摘要(Abstract)：

目的:建立一种测定豆豉中大豆异黄酮类成分含量的分析方法,并对黄豆及其发酵后豆豉中的大豆异黄酮含量进行比较。方法:采用反相高效液相色谱法,色谱柱Diamonsil-C18(250 mm×4.6 mm,5μm),保护柱Phenomenex-C18(4.6 mm×3 mm,5μm),流动相乙腈-0.2%磷酸(36∶64),流速为1.0 ml/min,柱温30℃,检测波长260 nm,测定黄豆及发酵后豆豉中大豆异黄酮成分大豆苷元和染料木素。结果:大豆苷元回归方程为Y=4.736 9X+23.332(r=0.999 6,n=5),在0.038 9～0.233 4μg范围呈良好线性关系;染料木素回归方程为Y=6.767 8X+4.2107(r=0.999 9,n=5),在0.039 9～0.239 1μg范围呈良好线性关系。结论:反相高效液相色谱法可用于豆豉中大豆异黄酮的含量测定,发酵后豆豉中大豆异黄酮的含量明显高于黄豆。
Objective:To establish a method for determination of soybean isoflavones(daidzein and genistin) in soybeans before and after fermentation with high performance liquid chromatography(HPLC),and to compare soybean isoflavone contents in them.Methods:The HPLC method was carried out on Diamonsil-C18(250 mm×4.6 mm,5 μm) chromatographic column and Phenomenex-C18(4.6 mm×3 mm,5 μm)protection column using acetonitrile-0.2% phosphoric acid(36∶64,V∶V)as mobile phase,and the flow rate was 1.0 ml/min.The column temperature was 30 ℃.Detection wavelength was 260 nm.Results:The regression equation of daidzein was Y=4.736 9X+23.332(r =0.999 6,n=5),and the calibration curve was linear in the range of 0.038 9 μg to 0.233 4 μg;The regression equation of genistin was Y=6.767 8X+4.210 7(r=0.999 9,n=5),and the calibration curve was linear in the range of 0.039 9 μg to 0.239 1 μg.Conclusions:The method is simple,effective,accurate and reproducible for determination of soybean isoflavones in soybeans and fermented soybeans.The contents of daidzein and genistin in fermented soybeans are obviously higher than those in un-fermented soybeans.

关键词(KeyWords)： 色谱法,高压液相;发酵;异黄酮类;黄豆
chromatography,high pressure liquid;fermentation;isoflavones;soybeans

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张敏,王鹏娇,舒阳,孟小夏,王沁,月清仪,高秀丽
ZHANG Min,WANG Pengjiao,SHU Yang,MENG Xiaoxia,WANG Qin,YUE Qingyi,GAO Xiuli (School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2011.04.010

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