施晓鋆,郭敬芝,黄绘,冯玉蓉,潘世春,文莉
SHI Xiao-yun; GUO Jing-zhi; HUANG Hui; FENG Yu-rong; PAN Shi-chun; WEN Li (Center for Reproductive Medicine;

• 1:贵阳市妇幼保健院生殖医学中心
• 2:贵阳市妇幼保健院生殖医学中心
• 3:贵阳市妇幼保健院生殖医学中心
• 4:贵阳市妇幼保健院生殖医学中心

摘要(Abstract)：

目的: 探讨卵子与精子短时间或长时间共培养对体外受精结局的影响。方法: 选择140个体外受精(IVF)周期作为研究对象,按精-卵共培养时间分为二组:A组(18h)共77个周期,B组(2h)共63个周期。B组在培养2h后将卵-冠-丘复合物移入新鲜的培养液中。将2组的受精率、卵裂率、优质胚胎率、胚胎植入率、临床妊娠率进行比较。结果: B组的受精率、优质胚胎率、胚胎植入率、临床妊娠率均显著高于A组,卵裂率无明显差别。结论: 长时间的精-卵共培养可能对受精及胚胎发育产生不利影响,缩短培养时间可以提高受精率、胚胎质量、胚胎植入率,继而提高临床妊娠率。
Objective: To investigate the effects of short time and long time sperm-oocyte coincubation on outcomes of in vitro fertilization (IVF). Methods: 140 IVF cycles were included in this study, and were divided into two groups according to different coincubation time. Group A (18h) contained 77 cycles; Group B (2h) contained 63 cycles. In group B, cumulus oophorus complexes were washed after 2-hours coincubation and moved into fresh culture medium. Those in group A were moved into fresh culture medium after 18-hours coincubation. The fertilization rates, cleavage rates, good embryo rates, implantation rates and clinical pregnancy rates of the 2 groups were compared. Results: The fertilization rate, good embryo rate, implantation rate and clinical pregnancy rate in group B were significantly higher than those in group A. Cleavage rates were not significantly different. Conclusions: Long time sperm-oocyte coincubation may produce negative effect on fertilization and embryo development. Shortening the coincubation time can increase clinical pregnancy rate by improving fertilization rate, embryo quality, and implantation rate.

关键词(KeyWords)： 受精,体外;合子;胚胎移植;共同培养;卵植入;妊娠率
fertilization in vitro;zygote;embryo transfer;coculture;ovum implantation;pregnancy rats

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 施晓鋆,郭敬芝,黄绘,冯玉蓉,潘世春,文莉
SHI Xiao-yun; GUO Jing-zhi; HUANG Hui; FENG Yu-rong; PAN Shi-chun; WEN Li (Center for Reproductive Medicine;

DOI: 10.19367/j.cnki.1000-2707.2005.02.014

参考文献(References)：

• [1]LGianaroli,AFiorentino,MCMagli,etal.Prolongedsperm oocyteexposureandhighspermconcentrationaf fecthumanembryoviabilityandpregnancyrate[J].HumReprod,1996,(11):2507-2511.
• [2]LGianaroli,MCristinaMagli,APFerraretti,etal.Reduc ingthetimeofsperm oocyteinteractioninhumanin vitrofertilizationimprovestheimplantationrate[J].HumRe prod,1996,(11):166-171.
• [3]MDirnfeld,DBider,MKoifman,etal.Shortenexposureofoocytestospermatozoaimprovesin vitrofertilizationout come:aprospective,randomized,controlledstudy[J].HumReprod,1999,14(10):2562-2564.
• [4]PMWasserman.Thebiologyofchemistryoffertilization[J].Science,1987,285:553-560.
• [5]AitkenRJ.AssessmentofspermfunctionforIVF[J].HumReprod,1988,3:89.
• [6]RRHenkel,WBSchill.SpermpreparationforART[J].ReproductiveBiologyandEndocrinology,2003,(1):108-130.
• [7]PMWasserman.Fertilizationinmammals[J].SciAm,1998,259:78-84.

文章评论(Comment)：