郑洋,潘娅,洪阳,向敏,王寒琪,王燕,陈腾祥
ZHENG Yang1,PAN Ya2,HONG Yang3,XIANG Min4,WANG Hanqi1,WANG Yan2,CHEN Tengxiang2(1.Department of Gastroenterology

• 1:贵阳医学院附院消化内科
• 2:贵阳医学院生理学教研室
• 3:贵阳市卫生局
• 4:贵阳医学院附院儿科

摘要(Abstract)：

目的:探讨干细胞相关基因Wnt5b和Nanog在阿霉素(ADM)作用下的人低转移性肝癌细胞系MH-CC97-L中的表达。方法:MHCC97-L细胞常规培养,用四甲基偶氮唑盐比色法(MTT)检测ADM作用72 h时MHCC97-L的半数致死量(LD50);蛋白质免疫印迹法(Western blot)检测ADM(LD50)作用下,干细胞相关基因Wnt5b和Nanog蛋白表达。结果:MTT实验结果显示,ADM对MHCC97-L的半数致死量(ID50)为0.423 3μmol/L;Western blot检测发现,在ADM(ID50)作用下,随作用时间延长,Wnt5b蛋白表达水平逐渐增高,12 h时达最高峰(P<0.05),以后则逐渐降低,72 h时降到最低(P<0.05);在ADM(ID50)作用下,随作用时间延长,Nanog蛋白表达水平逐渐降低,12 h时降到最低,以后则逐渐升高,72 h时达到最高(P<0.05)。结论:干细胞相关基因Wnt5b、Nanog在ADM作用下的人肝癌细胞系MHCC97-L中均有不同程度的表达。
Objective: To explore the expression of stem cell associated gene Wnt5b and Nanog in hepatocellular carcinoma cell line MHCC97-L incubating with adriamycin(ADM).Methods: MHCC97-L cells were routinely cultured.Median lethal dose(LD50) of MHCC97-L treated with ADM for 72 h were determined with MTT method.The expression of Wnt5b and Nanog proteins in MHCC97-L after treating with ADM(LD50) was detected with Western blot.Results: MTT results showed that LD50 of MHCC97-L treated with ADM was 0.423 3 μmol/L.The expression of Wnt5b increased when MHCC97-L was exposed to ADM within 4 hours,and reached peak at 12 hours,and then decreased gradually,and reduced to minimum at 72 h.When the incubation time was less than 12 h,Nanog decreased gradually with the time passing(P<0.05),and reduced to minimum at 12 h,and then it increased gradually,reached peak at 72 hours.Conclusions: Wnt5b and Nanog proteins express in MHCC97-L exposed to ADM in different degrees.

关键词(KeyWords)： 癌;肝细胞;阿霉素;干细胞;基因
carcinoma,hepatocellular;adriamycin;stem cell;gene

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81060176);; 贵阳市科技局社会发展领域科技攻关项目:筑科农字(2010)

作者(Author): 郑洋,潘娅,洪阳,向敏,王寒琪,王燕,陈腾祥
ZHENG Yang1,PAN Ya2,HONG Yang3,XIANG Min4,WANG Hanqi1,WANG Yan2,CHEN Tengxiang2(1.Department of Gastroenterology

DOI: 10.19367/j.cnki.1000-2707.2012.03.006

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