田静,许亚玲,许斌
TIAN Jing, XU Ya-ling, XU Bin(Drug Control Institute, Guizhou,Tongren 554300, China)

• 1:铜仁地区药品检验所
• 2:铜仁地区药品检验所
• 3:铜仁地区药品检验所 贵州铜仁554300
• 4:贵州铜仁554300

摘要(Abstract)：

目的:采用高效液相色谱法(HPLC)同时测定氨咖黄敏胶囊中对乙酰氨基酚、咖啡因、马来酸氯苯那敏的含量。方法:采用W aters Nova-pak C18(150 mm×3.9 mm,4μm)色谱柱,乙腈-0.03 mol/L磷酸氢二铵溶液为流动相,流速为1.0 m l/m in,检测波长214 nm,柱温30℃。结果:采用HPLC测定对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量,线性范围分别为205.6¹ 233.8μg,12.161 233.8μg,12.16⁷2.96μg,1.0372.96μg,1.03⁶.18μg,r=0.999 7,0.999 9,0.999 9;平均回收率分别为99.5%(RSD=0.3%),99.5%(RSD=0.5%),99.8%(RSD=0.8%)。结论:HPLC法简便、快速、准确,用此法同时测定氨咖黄敏胶囊中3个组成成分的含量,能更好地控制产品质量。
Objective: To determinate the contents of acetaminophen, caffeine, and chlorphenamine maleates in the Ankahuangmin Capsule with HPLC. Methods: The column used in the analysis was Waters Nova-pak C18(150mm×3.9mm,4μm) at 30℃, with the mobile phase of acetonitrile-0.03mol/L diammonium hydrogen phosphate (adjusted with phosphate acid to pH3.1) (12∶88) containing 0.75mmol/L heptane sulfonic acid sodium. The flow rate of the mobile phase was 1.0ml/min. The wavelength of the UV detector was 214nm. Result: The linear range of acetaminophen, caffeine, chlorphenamine maleates were 205.66.18μg,r=0.999 7,0.999 9,0.999 9;平均回收率分别为99.5%(RSD=0.3%),99.5%(RSD=0.5%),99.8%(RSD=0.8%)。结论:HPLC法简便、快速、准确,用此法同时测定氨咖黄敏胶囊中3个组成成分的含量,能更好地控制产品质量。
Objective: To determinate the contents of acetaminophen, caffeine, and chlorphenamine maleates in the Ankahuangmin Capsule with HPLC. Methods: The column used in the analysis was Waters Nova-pak C18(150mm×3.9mm,4μm) at 30℃, with the mobile phase of acetonitrile-0.03mol/L diammonium hydrogen phosphate (adjusted with phosphate acid to pH3.1) (12∶88) containing 0.75mmol/L heptane sulfonic acid sodium. The flow rate of the mobile phase was 1.0ml/min. The wavelength of the UV detector was 214nm. Result: The linear range of acetaminophen, caffeine, chlorphenamine maleates were 205.6¹233.8, 12.161233.8, 12.16⁷2.96, 1.0372.96, 1.03⁶.18μg/ml,and the coefficiency were r=0.9997, 0.9999,0.9999 respectively. The recovery rates were 99.5%(RSD=0.3%), 99.5%(RSD=0.5%), and 99.8%(RSD=0.8%)respectively. Conclusions: The HPLC method is simple, easy to operate, and can be used in quality control of Ankahuangmin Capsule.

关键词(KeyWords)： 醋氨酚;咖啡因;色谱法,高压液相;马来酸氯苯那敏;氨咖黄敏胶囊
acetaminophen; caffeine; chromatography,high pressure liquid; chlorphenamine maleates; ankahuangmin capsule

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 田静,许亚玲,许斌
TIAN Jing, XU Ya-ling, XU Bin(Drug Control Institute, Guizhou,Tongren 554300, China)

DOI: 10.19367/j.cnki.1000-2707.2005.04.008

参考文献(References)：

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