张天彪,赵滢,关一夫
ZHANG Tianbiao1,ZHAO Ying2,GUAN Yifu1(1.Department of Biochemistry and Molecular Biology

• 1:中国医科大学基础医学院生物化学与分子生物学教研室
• 2:中国医科大学附属盛京医院胃肠外科

摘要(Abstract)：

目的:研究胃肠道间质瘤(GIST)中p-ERK和PTEN蛋白的表达,并探讨二者之间的关系。方法:采用免疫组织化学方法检测124例GIST患者及癌旁正常组织中p-ERK和PTEN的表达水平,并进行比较分析。结果:GIST组织中p-ERK蛋白表达阳性率84.68%,明显高于正常组织(16.13%);PTEN蛋白表达的阳性率60.48%,明显低于正常癌旁组织(100%),差异有显著性(P<0.01);p-ERK和PTEN的表达与性别、年龄以及组织学类型无关,与NIH分级、淋巴结转移及浸润深度有关;p-ERK与PTEN表达呈负相关。结论:p-ERK的高表达、PTEN的低表达共同促进了GIST的发展。
Objective:To investigate the expression of p-ERK,and PTEN proteins in gastrointestinal stromal tumors (GIST) and their interrelation. Methods:p-ERK and PTEN expressions in GIST and normal tissues of 124 patients were detected by using immunohistochemistry method,and the results were analized. Results:The positive rate of p-ERK protein expression in GIST tissue (84.68%) was significantly higher than that in normal tissues (16.113%),while the positive rate of PTEN protein expression in GIST tissue (60.48%) was lower than that in normal tissue (100%) with statistically significance (P<0.01); Expressions of p-ERK and PTEN appeared to relate to NIH grading,lymphonode metastasis,and infiltration depth,but not to gender,age or tissue types. Expression of p-ERK and PTEN showed a negative correlation to each other. Conclusions:High expression of p-ERK,together with low expression of PTEN,propels the development of GIST,which may throw light to development of new anti-tumor drug and clinical tumor therapy.

关键词(KeyWords)： 胃肠肿瘤;有丝分裂素激活蛋白激酶激酶类;基因,肿瘤制;PTEN
gastrointestinal neoplasms; mitogen-activated protein kinase; genes,tumor suppressor; PTEN

Abstract:

Keywords:

基金项目(Foundation): 辽宁省教育厅科研基金资助项目(2009A726)

作者(Author): 张天彪,赵滢,关一夫
ZHANG Tianbiao1,ZHAO Ying2,GUAN Yifu1(1.Department of Biochemistry and Molecular Biology

DOI: 10.19367/j.cnki.1000-2707.2010.03.008

参考文献(References)：

• [1]Loesch M,Chen G.The p38MAPK stress pathway as a tumor suppressor or more[J].Front Biosci,2008(13):3581-93.
• [2]Steelman LS,Abrams SL,Whelan J,et al.Contributions of the Raf/MEK/ERK,PI3K/PTEN/Akt/mTOR and Jak/STAT pathways to leukemia[J]..Leukemia,2008(4):686-707.
• [3]Nezhat F,Datta MS,Hanson V,et al.The relationship of endometriosis and ovarian malignancy a review[J].Fertil Steril,2008(5):1559-1570.
• [4]Yin Y,Shen WH.PTEN:a new guardian of the genome[J].Oncogene,2008(41):5443-5453.
• [5]Wang X,Jiang X.PTEN:a default gate-keeping tumor suppressor with a versatile tail[J].Cell Res,2008(8):807-816.
• [6]Singer S,Rubin BP,Lux ML,et al.Prognostic value of KITmutation type,mitotic activity,and histologic subtype in gastrointestinal stromal tumors[J].J Clin Oncol,2002(18):3898-3905.
• [7]Lai JP,Sandhu DS,Shire AM,et al.The tumor suppres-sor function of human sulfatase1(SULF1)in carcinogen-esis[J].J Gastrointest Cancer,2008(1-4):149-58.
• [8]Muslin AJ.MAPK signalling in cardiovascular health and disease:molecular mechanisms and therapeutic targets[J].Clin Sci(Lond),2008(7):203-218.

文章评论(Comment)：