王雷鸣,梁冰,李淑芳,杨远兴,郝小燕,肖海涛,刘丽
WANG Leiming1,LIANG Bing1,LI Shufang1,YANG Yuanxing1,HAO Xiaoyan2,XIAO Haitao2,LIU Li3(1.Department of Pharmacology

• 1:贵阳医学院药理学教研室
• 2:贵阳医学院分析化学教研室
• 3:贵阳德昌祥药业有限公司

摘要(Abstract)：

目的:研究芪胶升白胶囊对小鼠耐缺氧和抗疲劳的作用。方法:芪胶升白胶囊分别以2.0 g/kg、1.0 g/kg、0.5 g/kg剂量灌胃各组小白鼠,每日一次,连续给药7 d;进行常压耐缺氧实验和化学性缺氧实验,观察小鼠窒息死亡时间,负重游泳实验观察小鼠窒息死亡时间,检测血中血红蛋白浓度、血乳酸含量、Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活力。结果:芪胶升白胶囊高、中、低剂量组能明显增加小鼠常压耐缺氧时间、化学性缺氧时间、负重游泳时间,增加负重游泳后血中血红蛋白浓度、血乳酸含量,增强Ca2+-Mg2+-ATP酶活力。结论:芪胶升白胶囊具有明显的耐缺氧作用和一定的抗疲劳作用,其作用可能与升高血红蛋白浓度和Ca2+-Mg2+-ATP酶活力以及降低血乳酸含量有关。
Objective: To explore the hypoxia tolerance and anti-fatigue effects of Qijiaoshenbai capsule(QSC) on rats.Methods: QSC at the dosages of 2.0,1.0 and 0.5 g/kg were administered by gavage once a day for 7 days.Anti-hypoxia experiment under normal pressure,chemical anti-hypoxia experiment and load swimming experiment were carried out and suffocation time of the rats was observed.The blood hemoglobin(Hb)concentration,blood lactic acid(BLA)content and the activity of Ca2+-Mg2+-ATPase and Na+-K+-ATPase of load swimming mice were measured.Results: High,moderate and low dosages of QSC prolonged the survival time of hypoxia mice under normal pressure and mice exposed to sodium nitrite,and prolonged swimming time of load mice.The Hb concentration increased,the activity of Ca2+-Mg2+-ATPase enhanced,and BLA content decreased in the three QSC groups.Conclusions: QSC has obvious anti-hypoxia effects and certain anti-fatigue effects,which may be related to the increase of Hb concentration and the activity of Ca2+-Mg2+-ATPase and the decrease of BLA content.

关键词(KeyWords)： 芪胶升白胶囊;缺氧;疲劳
Qijiaoshenbai capsule;anoxia;fatigue

Abstract:

Keywords:

基金项目(Foundation): 贵州省中药现代化科技产业研究开发专项项目:黔科合中药字2010[5005]

作者(Author): 王雷鸣,梁冰,李淑芳,杨远兴,郝小燕,肖海涛,刘丽
WANG Leiming1,LIANG Bing1,LI Shufang1,YANG Yuanxing1,HAO Xiaoyan2,XIAO Haitao2,LIU Li3(1.Department of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2012.03.010

参考文献(References)：

• [1]罗莉,玉崧成,王金水,等.大枣多糖结构及药理活性的研究进展[J].安徽农业科学,2010(30):16860-16861.
• [2]陶静,范欣生,张欢.黄芪治疗放疗化疗后白细胞减少症的应用分析[J].中医药学报,2010(6):67-70.
• [3]张旭,张汝学,涂宏海,等.中药复方提高机体耐缺氧能力及机制的研究进展[J].中华中医药学刊,2007(9):1824-1827.
• [4]陈玉满,陈江,毛光明,等.高山红景天的耐缺氧作用实验研究[J].中国卫生检验杂志,2007(8):1494-1495,1510.
• [5]于海玲,李华伟,李雪,等.复方黄芪多糖对小鼠的抗疲劳和耐缺氧作用[J].延边大学医学学报,2009(3):160-162.
• [6]Tan W,Yu KQ,Liu YY,et al.Anti-fatigue activity of polysaccharides extract from Radix Rehmanniae Preparata [J].Int J Biol Macromol,2012(1):59-62.
• [7]方伟进,李艳,曹珊珊,等.山茱萸果核提取物耐疲劳和耐缺氧作用研究[J].河南科技大学学报(医学版),2011(3):167-169.
• [8]张顺斌.原发性高血压肾病患者红细胞ATP酶活性检测的临床意义[J].淮海医药,2008(6):477-478.

文章评论(Comment)：