贵州医科大学学报

2007, No.125(02) 137-139

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

海洛因依赖大鼠胰岛D细胞免疫组织化学研究
Immunohistochemical Study on D Cells in Panceatic Islet of Heroin Dependent Rats

韩晶;梁文妹;
HAN Jing,LIANG Wenmei(Department of Histo-Embryology,Guiyang Medical college,Guiyang 550004,China)

摘要(Abstract):

目的:探讨海洛因依赖对大鼠胰岛生长抑素(somatostatin,SS)免疫反应(IR)细胞(D细胞)的影响。方法:用免疫组织化学SABC单染法、形态计量法,观察海洛因依赖大鼠胰岛SS-IR细胞免疫组织化学反应及平均灰度值的变化。结果:与正常组和盐水对照组比较,海洛因依赖大鼠胰岛SS-IR细胞免疫染色增强,平均灰度值降低,差异有显著性。结论:胰岛SS-IR细胞参与了海洛因依赖期间机体的调节过程。
Objective: The morphological and functional changes of somatostatin immunoreactive(SS-IR)cells(D cells) in pancreatic islets of rats were studied to investigate the effect of heroin dependence on the secretion of SS-IR cells.Methods: The heroin dependent model of rats was made by subcutaneous injection of heroin.The immunohistochemical SABC staining method and image analysis were adopted in the studies.Results: The immunological staining was obviously stronger,and mean grey degree was remarkably lower in SS-IR cells of rats in heroin dependent group than that in rats of normal control group.Conclusions: By decreasing the secretion of SS,the SS-IRcells of islet involved in body regulation during the heroin dependence process.

关键词(KeyWords): 海洛因依赖;胰岛;生长抑素;细胞;免疫组织化学,大鼠
heroin dependence;islet of langerhans;somatostatin;cells;immunohistochemistry;rats

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技教育人才省长专项资金资助项目,黔科教办[2001-3]

作者(Author): 韩晶;梁文妹;
HAN Jing,LIANG Wenmei(Department of Histo-Embryology,Guiyang Medical college,Guiyang 550004,China)

Email:

DOI: 10.19367/j.cnki.1000-2707.2007.02.009

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享