贵州医科大学学报

2008, No.135(06) 632-634+639

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大鼠整体低氧预处理减轻肺缺血再灌注损伤
An Experimental Study on the Protective Effect of Whole-body Hypoxic Preconditioning on Lung Injury Caused by Ischemic Reperfusion in Rats

李涛,陈莹,李保罗,陆德琴
LI Tao,CHEN Ying,LI Baoluo,LU Deqin (Department of Pathophysiology

摘要(Abstract):

目的:探讨低氧预处理(HPC)对肺缺血再灌注(I/R)损伤的保护作用。方法:重复性低氧5次建立大鼠HPC模型,双活结套扎左侧肺门45min后再灌注120min复制肺I/R损伤模型;设立对照组、I/R组和HPC+I/R组,每组10只大鼠,检测各组肺组织湿重与干重比,肺组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)及髓过氧化物酶(MPO)活性。结果:I/R组大鼠肺组织湿重与干重比较对照组明显增加(P<0.05),肺组织SOD活性显著降低(P<0.01),MDA含量和MPO活性明显增高(P<0.05);HPC+I/R组大鼠上述指标较I/R组均明显改善,差异有显著性。结论:大鼠整体HPC可能通过减轻氧化应激反应,保护肺的缺血再灌注损伤。
Objective:To investigate the protective effect of hypoxic preconditioning (HPC) on lung ischemia-reperfusion injury in rats. Methods:HPC model was established with 5 times of repetitive sublethal hypoxia. Thirty rats were randomly divided into the following three groups (n=10 in each):control group,ischemia-reperfusion injury group (group I/R),and group HPC+I/R. I/R injury model rats underwent 45 min of left hilar ligation followed by 120 min of reperfusion. The ratio of wet/dry weight of lung tissue was measured and calculated. The contents of malondialdehyde (MDA),and activities of superoxide dismutase (SOD) and myeloperoxidase (MPO) in lung tissue were determined with spectrophotometry. Results:In group I/R,the wet/dry weight ratio of lung was significantly higher than that of control group (P<0.05),and the MDA content and MPO activity in lung tissue were dramatically higher (P<0.05),SOD activity was significantly lower (P<0.01) than those of control group. In group HPC+I/R,that rats subjected to HPC,these oxidative stress indexes were significantly improved when rats exposed to ischemia-reperfusion.Conclusion:Whole-body hypoxic preconditioning in rats can protect lung from I/R injury by reducing oxidative stress.

关键词(KeyWords): 缺血;肺;再灌注损伤;低氧预处理;大鼠,Sprague-Dawley
ischemia; lungs; reperfusion injury; hypoxic preconditioning; rats,sprague-dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省省长基金资助项目(S2003-9)

作者(Author): 李涛,陈莹,李保罗,陆德琴
LI Tao,CHEN Ying,LI Baoluo,LU Deqin (Department of Pathophysiology

DOI: 10.19367/j.cnki.1000-2707.2008.06.019

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