刘宓,许晶,李海志,毕晓洁,何玉,汪峰,潘卫
LIU Mi1,XU Jing2,LI Haizhi2,BI Xiaojie1,HE Yu1,WANG Feng1,PAN Wei1(1.Department of Inspection

• 1:贵阳医学院检验系
• 2:贵阳医学院附属贵州肿瘤医院
• 3:贵阳市第一中学

摘要(Abstract)：

目的:建立HPLC测定铜钱草中残留抗生素诺氟沙星含量的方法。方法:以贵阳市某医院花坛内铜钱草为研究对象,采用Diamonsil C18(4.6 mm×250 mm,5μm)色谱柱,流动相为甲醇-1%醋酸(16∶84),流速1.0 mL/min,检测波长282 nm,柱温30℃。结果:诺氟沙星在1.049 6×10-3～104.96×10-3μg(r=0.999 9)具有良好的线性关系,诺氟沙星检出限达0.005 7 mg/L,平均回收率为96.6%,相对标准偏差(RSD)为2.41%。结论:HPLC法操作简便,测定结果准确,可作为环境中残留抗生素诺氟沙星含量的检测方法。
Objective: To establish an high performance liquid chromatography(HPLC) method for quantitative determination of norfloxacin residual volume in Hydrocotyle vulgaris.Methods: Hydrocotyle vulgaris in a flower bed of some hospital in Guiyang was selected as study subject.The HPLC method was carried out on Diamonsil C18(4.6 mm×250 mm,5 μm) using methanol-1% acetic acid(16∶84) as mobile phase.The flow rate was 1.0ml/min.Detection wavelength was 282 nm.The column temperature was 30℃.Results: The calibration curve for norfloxacin was linear in the range of 1.049 6×10-3~104.96 ×10-3μg(r=0.999 9).The detection limit was 0.005 7 μg/mL.The average recovery rate was 96.6%(RSD 2.41%).Conclusions: The method is simple and accurate for quantitative determination of norfloxacin residual volume in environment.

关键词(KeyWords)： 色谱法;高压液相;铜钱草;诺氟沙星;抗生素类
chromatography,high pressure liquid;Hydrocotyle vulgaris;norfloxacin;antibiotics

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金[黔科合J字(2011)2275号]

作者(Author): 刘宓,许晶,李海志,毕晓洁,何玉,汪峰,潘卫
LIU Mi1,XU Jing2,LI Haizhi2,BI Xiaojie1,HE Yu1,WANG Feng1,PAN Wei1(1.Department of Inspection

DOI: 10.19367/j.cnki.1000-2707.2012.03.011

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