贵州医科大学学报

2005, (06) 502-503+506

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一种适于AFLP分析的天麻花葶DNA提取方法
An Extraction Method of Genome DNA from Gastrodia elata Scape for AFLP Analysis

常楚瑞;王晓丽;
CHANG Chu-rui,WANG Xiao-li(Botany and Pharmacognosy Department,School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

摘要(Abstract):

目的:获得能用于中药天麻扩增片段长度多态性(AFLP)分析及其它分子生物学研究的高质量DNA。方法:以天麻花葶为材料,采用改良SDS法提取天麻DNA,经紫外分光光度计和琼脂糖凝胶电泳检测。结果:改良的SDS法能获得高质量的DNA,OD260/OD280为1.751.9,蛋白质、多糖、RNA等去除较彻底,适于AFLP分析。结论:改良的SDS法所提取天麻DNA适于AFLP分析。
Objective: TO extract high-quality DNA from Chinese traditional medicine Gastrodia elata for AFLP and other molecular biological analysis.Methods: Improved SDS method was used to extract DNA.The obtained DNA was examined with ultraviolet absorption test and agarose gel electrophoresis.Results: The purity of the extracted DNA was very high with OD_(260)/OD_(280) values of 1.75~1.9.The protein,polyhexose and RNA were elminated completely. Conclusions: The improved SDS method was suitable for extracting DNA from Gastrodia elata for AFLP analysis.

关键词(KeyWords): 天麻;核酸扩增技术;基因组,植物
Rhizoma Gastrodiae;nucleic acid amplification techniques;genome,plant

Abstract:

Keywords:

基金项目(Foundation): 国家科技部2004BA721A33、黔科合农社字(2001)1125号、黔科合中药专字(2003)51号

作者(Authors): 常楚瑞;王晓丽;
CHANG Chu-rui,WANG Xiao-li(Botany and Pharmacognosy Department,School of Pharmacy,Guiyang Medical College,Guiyang 550004,China)

DOI: 10.19367/j.cnki.1000-2707.2005.06.007

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