葛勤,刘同华,张恩娟,黄林清
GE Qin; LIU Tong-hua; ZHANG En-juan; HUANG Lin-qing (Xinqiao Hospital;

• 1:第三军医大学新桥医院
• 2:第三军医大学新桥医院
• 3:第三军医大学新桥医院
• 4:第三军医大学新桥医院 重庆400037

摘要(Abstract)：

目的 :用高效液相色谱法 (HPLC)测定灯盏花素注射剂中灯盏花乙素的含量。方法 :以HypersilBDSC18柱为色谱柱 ;流动相为甲醇 磷酸盐缓冲液 (35∶6 5 ,V/V ,pH =3 0 ) ,流速 1 0ml/min ;检测波长为 335nm。结果 :线性关系好 ,r=0 9998,平均回收率为 10 0 6 5 % ,高、中、低 3种浓度日内精密度RSD在 1 2 2 %～ 2 99% ,日间精密度RSD在 2 32 %～ 4 82 %。结论 :HPLC法简便、准确 ,适用于灯盏花素注射剂的质量检验分析
Objective: To study the determination method of scutellarin in breviscapine injection using HPLC. Method: The separation was performed on a Hypersil BDS C 18 column. The mobile phase was composed of methanol-phosphate buffer (35∶65, V/V, pH=3.0). The ultraviolet wavelength was set at 335 nm. Results: A good linearity (r=0.999 8) was obtained. The mean recovery rate was 100.65%. The within-day RSD was between 1.22% and 2.99%, and inter-day RSD was between 2.32% and 4.82%. Conclusion: The method is simple, convenient and accurate and can be used for the quality control of breviscapine injection.

关键词(KeyWords)： 植物制剂;黄酮类;色谱法,高压液相;灯盏花素注射剂;乙素
plant preparations; flavones; chromatography,high pressure liquid; breviscapine injection; scutellarin

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 葛勤,刘同华,张恩娟,黄林清
GE Qin; LIU Tong-hua; ZHANG En-juan; HUANG Lin-qing (Xinqiao Hospital;

DOI: 10.19367/j.cnki.1000-2707.2004.03.007

参考文献(References)：

• [1]王桂霞.灯盏花素药理及临床应用[J].时珍国医国药,1999,10(8):639.
• [2]杨菊妹.灯盏花素临床应用进展[J].中国药业,2000,9(12):64-65.
• [3]陈丽,画红顺.温度对灯盏花素注射剂含量的影响[J].时珍国医国药,1999,10(12):906.

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