吴运莉,舒阳,孟小夏,张敏,王鹏娇,高秀丽
WU Yunli,SHU Yang,MENG Xiaoxia,ZHANG Min,WANG Pengjiao,GAO Xiuli (School of Pharmacology

• 1:贵阳医学院药学院

摘要(Abstract)：

目的:建立一种测定香青百草油中丁香酚含量的方法。方法:采用高效液相色谱法,色谱柱Dia-monsil-C18(250 mm×4.6 mm,5μm),流动相为甲醇-水(65∶35),流速为1.0 ml/min,柱温为25℃,检测波长为280 nm。结果:丁香酚在0.034～0.549μg范围呈良好线性关系,平均回收率为97.63%,RSD为1.22%(n=9)。结论:高效液相色谱法简便、快速、准确、重复性好,可用于香青百草油中丁香酚的含量测定。
Objective: To establish a method for content determination of eugenol in Xiangqingbaicao oil.Methods: The HPLC method was carried out on Diamonsil-C18(250 mm×4.6 mm,5 μm) column using methanol-water(65∶35) as mobile phase,and flow rate was 1.0ml/min.Temperature of the column was 25 ℃.Detection wavelength was 280 nm.Results: The calibration curve for eugenol was linear in the range of 0.034～0.549 μg.The average recovery was 97.63%,and RSD was 1.22%(n=9).Conclusions: The method is simple,effective,accurate and reproducible for content determination of eugenol in Xiangqingbaicao oil.

关键词(KeyWords)： 色谱法,高压液相;香青百草油;丁香酚
chromatography,high pressure liquid;Xiangqingbaicao oil;eugenol

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 吴运莉,舒阳,孟小夏,张敏,王鹏娇,高秀丽
WU Yunli,SHU Yang,MENG Xiaoxia,ZHANG Min,WANG Pengjiao,GAO Xiuli (School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2011.05.014

参考文献(References)：

• [1]曾惠芳,黄耀海,李湘力,等.百草油中薄荷脑、肉桂醛、丁香酚的含量测定[J].中成药,2007(29):558-560.
• [2]王海英.气相色谱法测定丁香油中丁香酚含量[J].哈尔滨医药,2009(29):20-21.
• [3]王喆桅,金城,焦懿,等.气相色谱法测定行军散中丁香酚的含量[J].解放军药学学报,2010(26):137-138.
• [4]武爱玲,赵新杰.气相色谱法测定接骨止痛酊中水杨酸甲酯和丁香酚的含量[J].中医药报,2011(17):75-76.
• [5]牛瑞仙.高效液相色谱法测定八味檀香散中丁香酚的含量[J].泰山医学院学报,2009(30):695-696.
• [6]张少彦,饶华孟.高效液相色谱法测定丁硼乳膏中丁香酚的含量[J].海峡药学,2009(10):41-42.
• [7]吴军侠,赵红侠.高效液相色谱法测定蒙药暖宫七味散中丁香酚含量[J].中国业,2010(17):31.
• [8]王也,裘国丽,黄华.高效液相色谱法测定丁桂儿脐贴中丁香酚和桂皮醛含量[J].中国药业,2011(20):43.
• [9]谢友良,曾宝,吴燕红,等.HPLC法测定参香养胃胶囊中橙皮苷、丁香酚和厚朴酚的含量测定[J].中药新药与临床药理,2006(1):12-14.

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