张东兴,颜登国,赵丙波
ZHANG Dongxing,YAN Dengguo,ZHAO Bingbo(Department of General Surgery

• 1:贵阳医学院附院普外科
• 2:贵阳医学院
• 3:贵州电力医院

摘要(Abstract)：

目的:建立人大肠癌裸鼠皮下移植瘤模型的方法。方法:从新鲜的人大肠癌标本上切取肿瘤组织,移植于裸鼠皮下,原代移植成功后再进行传代,观察裸鼠摄食、活动状况、成瘤时间、成瘤率、潜伏期、肿瘤生长情况、移植瘤大小及组织病理学变化。结果:原代肿瘤移植成功率为40%,传代肿瘤移植成功率为75%,原代移植肿瘤及传代移植肿瘤组织形态学特征均与患者原发肿瘤保持一致。结论:初步建立了一种有效的人大肠癌裸鼠皮下移植瘤模型,为大肠癌的研究奠定了基础。
Objective:To study methods for establishment of subcutaneous transplantation models of human colorectal carcinoma with nude mice. Methods:Fresh tissues of colorectal carcinoma were collected,and transplanted into subcutaneous places of nude mice. The successfully transplanted primary generation tumor was then passed down to further generations. Feeding and activity status of tumor-bearing mice,formation time,survival rate,latency,growth and size of the tumor were observed. Histo-phathological changes of tumor were checked. Results:Survival rate was 40% for primarily transplanted tumor,and was 75% for passage tumors. The morphological characteristics of primary tumor and further generations of tumors were consistent with those of original tumor. Conclusion:The subcutaneously xenotransplanted tumor model of human colorectal carcinoma with nude mice has been successfully established,which provides a basis for individualization studies of colorectal carcinoma patients.

关键词(KeyWords)： 癌;大肠;肿瘤移植;疾病模型,动物;小鼠,裸
carcinoma; intestine,large; neoplasm transplantation; disease models,animal; mice,nude

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张东兴,颜登国,赵丙波
ZHANG Dongxing,YAN Dengguo,ZHAO Bingbo(Department of General Surgery

DOI: 10.19367/j.cnki.1000-2707.2010.03.011

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