喻南慧,黄勇攀,罗俊
YU Nanhui,HUANG Yongpan,LUO Jun(Department of Pharmachology

• 1:贵阳医学院药理学教研室
• 2:贵阳医学院

摘要(Abstract)：

目的:探讨灵芝三萜类化合物(GLT)对阿尔茨海默病(AD)的防治作用,为临床新药的开发和应用提供理论依据。方法:将经Morris水迷宫筛选合格的SD大鼠80只分为8组,正常对照组、假手术组,模型组、GLT低、中、高(GLT0.25、0.5、1.0g/kg)剂量组、阳性药组(健脑胶囊组0.54g/kg)和溶媒组(食用油10ml/kg),除正常对照组和假手术组外,其余各组大鼠经侧脑室注射Aβ25-352μl制备AD大鼠模型,正常对照组侧脑室注射等量生理盐水,术后次日开始灌胃给药。每天1次,连续给药20d后,处死动物取脑组织制备匀浆。采用光化学法检测脑匀浆中过氧化氢酶(CAT)、乙酰胆碱酯酶(ACHE)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果:与正常对照组比较,模型组脑组织中CAT活性降低,ACHE活性升高,SOD活性降低,MDA含量升高。与模型组比较,三个GLT给药组脑组织中,CAT活力均升高,ACHE活力均下降,SOD活性均升高,MDA含量均降低。结论:灵芝三萜类化合物对AD模型大鼠具有增强清除自由基的能力,抗脂质过氧化及促进胆碱能神经系统功能的恢复。
Objective:To investigate the preventive effects of ganodermo lucidum triterpenoids (GLT) to Alzheimer disease(AD)in model rats. so as to provide theory basis for new drug exploitation and clinical application. Methods:Eighty SD rats chosen by Morris water maze test were randomly divided into 8 groups:normal control group ( group NC,received 10 ml/kg of distilled water),sham operation group (group SO,received 10 ml/kg of distilled water),model group (group M,received 10ml/kg of distilled water),GLT treated groups (group G1,received 0.25 g/kg of GLT; group G2,0.50 g/kg,and group 3,1.0 g/kg),masculine drug group (group MD,received 0.54 g/kg of Jiannao capsules),and solute mediated group (group SM,received 10 ml/kg of edible oil). All of the SD rats except those in group NC and group SO were microinjected with beta-amyloid peptide segment 25-35 (Aβ25-35) into lateral ventricle to produce AD model,and instead,rats in group NC were injected with saline. GLT treatment began on the next day,once a day intragastrically for 20 days. Then,the animals were executed,and the brain tissues were taken out and blended for the tests of activities of catalase (CAT) ,acetyl cholinesterase (ACHE),superoxide dismutase (SOD),and the content of malondialdehyde (MDA) with chemistry detecting kits. Results:The activities of CAT,SOD were lower,while ACHE activity and MDA content were higher in rats of group M than those in group NC; The activities of CAT,SOD were higher,while ACHE activity and MDA content were lower in GLT groups than those in group M. Conclusion:The GLT has ability of strengthening the cleaning of free radicals in Aβ25-35 toxicated rats,and ability of anti-liqid-peroxidation,and can promote the restore of choline nervous system function.

关键词(KeyWords)： 阿尔茨海默病;灵芝属;脑;过氧化氢酶;乙酰胆碱脂酶;超氧化物歧化酶;丙二醛;大鼠,Sprague-Dawley
Alzheimer disease;Ganoderma; brain; catalase; acetylcholinesterase; superoxide dismutase; malondialdehyde; rat,sprague-dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技攻关项目[D2004-4]

作者(Author): 喻南慧,黄勇攀,罗俊
YU Nanhui,HUANG Yongpan,LUO Jun(Department of Pharmachology

DOI: 10.19367/j.cnki.1000-2707.2008.06.021

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