康哲,张春林,陈汉彬
KANG Zhe,ZHANG Chunlin,CHEN Hanbin(Department of Biology

• 1:贵阳医学院生物学教研室

摘要(Abstract)：

目的:克隆致倦库蚊Cecropins基因序列,并进行生物信息学分析。方法:选取致倦库蚊成虫,提取其DNA并以此为模板,设计一对引物,采用PCR技术扩增出Cecropins基因,应用相关生物信息学软件分析其序列。结果:克隆出致倦库蚊Cecropins基因,并测定其扩增产物序列,经同源对比确认,进行了相关序列分析。结论:获得致倦库蚊Cecropins基因序列,基因长度为259bp,与尖音库蚊一致性为97%。
Objective:To clone and analyze the sequence of cecropin gene of Culex quinquefasciatus. Methods:Genomic DNA of C. quinquefasciatus adults was isolated,and was used as template to clone cecropin gene by PCR with a pair of designed primers ATGAACTTCAACAAGCTTT and CAAGTCCTACT TTCCAAGT. The obtained sequence was analyzed with bioinformatics softwares.Results:A cecropin gene of C. quinquefasciatus was obtained,and was sequenced and analyzed. The results showed that the gene was 259 bp and was 97% consistent with that of cecropin gene of C. pipiens. Conclusion:The cloning is successful,and one of the cecropin genes of C. quinquefasciatus was obtained.

关键词(KeyWords)： 库蚊属;基因cecropin;基因顺序
Culex; gene cecropin; gene order

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金资助项目[黔科合丁字(2006)2077号]

作者(Author): 康哲,张春林,陈汉彬
KANG Zhe,ZHANG Chunlin,CHEN Hanbin(Department of Biology

DOI: 10.19367/j.cnki.1000-2707.2008.06.022

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