吴珊,董为伟,董佑忠
WU Shan 1; DONG Wei-wei 2; DONG You-zhong 1 (1. Department of Neurology;

• 1:贵阳医学院附院神经科
• 2:重庆医科大学神经病学研究所
• 3:贵阳医学院附院神经科 贵州贵阳550004
• 4:重庆400046

摘要(Abstract)：

目的 :了解缺氧 复氧条件下内皮细胞超氧化物歧化酶 (SOD)活性的变化及对内皮 中性粒细胞黏附的影响。方法 :体外培养人脐静脉内皮细胞 ,制造缺氧及再供氧模型 ,观测SOD活性变化及内皮 中性粒细胞黏附率 ,并在复氧时进行干预。结果 :1.内皮细胞的SOD活性在缺氧 90min后明显下降。复氧后 4h仍然继续降低 ,与缺氧 90min组比较有显著性差异。 2 .复氧同时加入抗氧化剂N 乙酰半胱氨酸 (NAC) ,4h后测SOD活性与缺氧组比较差异无统计学意义 ,但高于复氧组 ;复氧同时加入细胞因子白介素 1β(IL 1β) ,4h后内皮细胞SOD活性与复氧组差异无显著性 ;但复氧同时加入肿瘤坏死因子 α(TNF α)后SOD活性比复氧组明显下降。 3.内皮 白细胞黏附率在缺氧组和复氧组均高于正常对照组 ,但二者之间没有显著性差异。 4 .复氧同时加入NAC ,对内皮 中性粒细胞黏附率无明显抑制 ;但复氧同时加入细胞因子IL 1β或TNF α后内皮 中性粒细胞黏附率明显高于复氧组。结论 :1.内皮细胞缺氧 90min后 ,其内源性SOD活性下降 ,复氧后 4h加重SOD活性抑制。 2 .复氧同时加入外源性抗氧化剂NAC可对抗复氧所致SOD活性下降 ;外源性IL 1β对此无影响 ;外源性TNF α促进复氧所致SOD活性抑制。 3.内皮 中性粒细胞的黏附在缺氧时加强 ,而复氧 4
Objective: Production of reactive oxygen species (ROS) by ischemic tissue after ischemia-reperfusion is an important factor which contribute to tissue injury. SOD as an special enzyme could eliminate ROS. Inflammation is another way to damage reperfusion tissue. Endothelium-neutrophil adhesion is one of the key steps. The objective of this research was to determine whether the SOD activity of endothelial cell after anoxia-reoxygenation would be changed and the relationship between SOD activity and endothelium-neutrophil adhesion.Methods: Endothelial cells were harvested from umbilical cord and cultured in vitro. Confluent HUVECS monolayer were exposed to anoxia for 90min, then reoxygenated for 4 h. As a part of the experiment, IL-1β, TNF-α, and N-acetyl-L-cysteine (NAC) were used respectively at the moment of reoxygenation.Results: SOD activity decreased after anoxia and continued to decline after 4h of reoxygenation. NAC reserved the SOD activity after reoxygenation, but TNF-α damaged it. IL-1β had no effect on it. The adhesive rate of neutrophil to endothelium increased after anoxia and reoxygenation. NAC had no effect on it, but IL-1β,TNF-α intensified it.Conclusions: SOD activity of endothelium decreases after anoxia and reoxygenation. At the moment of reoxygenation, NAC benefits SOD activity but TNF-α damages SOD activity. IL-1β has no effect on SOD activity. The adhesive rate of neutrophil to endothelium increases after anoxia and reoxygenation. At the moment of reoxygenation, NAC has no effect on the adhesive rate, but IL-1β、TNF-αcan enhance it. There is no relationship between SOD activity of endothelium and endothelium-neutrophil adhesion.

关键词(KeyWords)： 超氧化物歧化酶;内皮;缺氧;脐静脉;中性粒细胞
superoxide dismutase; endothelium; anoxia; umbilical veins; neutrophil

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术厅资助项目 (黔基合计字 1 999 30 78)

作者(Authors): 吴珊,董为伟,董佑忠
WU Shan 1; DONG Wei-wei 2; DONG You-zhong 1 (1. Department of Neurology;

DOI: 10.19367/j.cnki.1000-2707.2004.05.005

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