潘雪莉,魏绍峰,张方倩,张博
PAN Xueli,WEI Shaofeng,ZHANG Fangqian,ZHANG Bo (School of Public Health

• 1:贵阳医学院公共卫生学院

摘要(Abstract)：

目的:从体内和体外2个试验体系检测95%氨氯吡啶酸原药的致突变性,预测其遗传危害,为其安全使用提供科学依据。方法:采用小鼠骨髓嗜多染红细胞微核试验、中国仓鼠肺细胞(CHL)染色体畸变试验方法检测95%氨氯吡啶酸原药的致突变性。结果:95%氨氯吡啶酸原药各剂量组小鼠骨髓嗜多染红细胞微核率与阴性对照组比较,差异无统计学意义(P>0.05);95%氨氯吡啶酸原药各剂量组CHL细胞染色体畸变率,在直接作用和代谢活化条件下,与溶剂对照组比较,差异均无统计学意义(P>0.05)。结论:在本试验条件下,95%氨氯吡啶酸原药无诱导小鼠骨髓细胞染色体损伤及致CHL细胞染色体畸变的作用。
Objective:To observe the mutagenicity of 95% picloram both in vivo and in vitro,and to predict its genetic damage,and so as to provide scientific basis for its safe use.Methods:Mouse bone marrow polychromatic erythrocytes(PCE) micronucleus test and chinese hamster lung cell(CHL) aberration test were used for the observation of the mutagenicity of 95% picloram.Results:There was no significant difference of micronucleus rates in mouse bone marrow PCE between groups exposed to different doses of 95% picloram and negative control group(P>0.05),and there was no significant difference of chromosomal aberration rates in CHL cells between different dose groups and solvent control group(P>0.05)neither in direct effect nor in metabolic activation conditions.Conclusions:Under this experimental condition,95% picloram can not induce chromosome injury of mouse bone ma-rrow PCE or chromosomal aberration of CHL cells.

关键词(KeyWords)： 农药;氨氯吡啶酸;微核;染色体畸变;小鼠
pesticides;aminopyralid;micronucleus;chromosomal aberration;mice

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 潘雪莉,魏绍峰,张方倩,张博
PAN Xueli,WEI Shaofeng,ZHANG Fangqian,ZHANG Bo (School of Public Health

DOI: 10.19367/j.cnki.1000-2707.2011.04.006

参考文献(References)：

• [1]陈贤均,赵祖国.我国农产品农药残留现状及农药遗传毒性研究进展[J].毒理学杂志,2010(4):324-327.
• [2]杨卫东,曲俊山,尹国龙,等.24%氨氯吡啶酸水剂防治林地阔叶杂草试验[J].森林工程,2006(3):8-11.
• [3]OECD,OECD Guidelines for the Testing of Chemicals,474.Mammalian Erythrocyte Micronucleus Test[EB/OL][1997-07-21].
• [4]OECD,OECD Guidelines for the Testing of Chemicals,473.In vitro Mammalian Chromosome Aberration Test[EB/OL][1997-07-21].
• [5]王心如.毒理学基础[M].5版.北京:人民卫生出版社,2007(5):1-152.
• [6]何林,吴赤蓬,邹志飞.亚硝酸钠对中国仓鼠肺细胞染色体畸变率的影响[J].现代预防医学,2008(17):3288-3293.
• [7]罗鹏,张爱华,潘雪莉.阿嗪米特致中国仓鼠肺细胞体外染色体畸变试验[J].实用临床医药杂志,2009(13):63-64.

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