支原体培养基的改良An improved Culture Medium for Mycoplasma
唐七义,王和
Tang Qiyi, et al Department of Microbiology
摘要(Abstract)：
以1％蛋黄和5％蛋清代替血清制成培养基培养支原体和在培养基中加入溴甲酚紫作pH指示剂观察其生化反应。结果表明,支原体生长迅速且菌落形态典型,溴甲酚紫变色pH阈较低,能更确切地反映支原体生长和代谢活动。莱氏支原体能发酵葡萄糖和乳糖产酸,能还原TTC。但不发酵蕈糖和不吸附红细胞。
In order to simplify the mycoplasma culture medium preparation, an egg medium contained 1% yolk and 5% egg white was made. Bromoresol purple was substituted for phenol red as a pH indicator. On this medium, acholeplasma laidlawii grew well, formed typical colony, fermented glucose and lactose, but not trehalose dihydrate. It also reduced TTC, but not be absorbed to chick red blood cell.
关键词(KeyWords)：
支原体属;培养基;卵白;蛋黄
mycoplsma;culture medium;egg white;egg yolk
基金项目(Foundation):
作者(Author):
唐七义,王和
Tang Qiyi, et al Department of Microbiology
DOI: 10.19367/j.cnki.1000-2707.1992.04.016
参考文献(References)：
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- 2 李宁丽,等.解脲支原体固体培养基改良.蚌埠医学院学报,1989,14(1) :19-20
- 3 王和.L型鸡蛋培养基的制备和细菌L型的诱导.贵阳医学院学报,1988,13(1) :42-46
- 4 李仲兴,等主编.临床细菌学.第一版.北京:人民卫生出版社,1986． 381-382
- 5 McElhaney, R N. The structure and function of the acholeplasma laidlawii plasma membrane. Biochemical et Biophysical Acta, 1984, 779:1-42
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