余鸿标;姚吉龙;
YU Hongbiao;YAO Jilong;Department of Gynecology,Luohu hospital of Shenzhen;Department of Gynecology,Maternal and Child Health Hospital of Shenzhen;

• 1:深圳市罗湖医院妇科
• 2:深圳市妇幼保健院妇科

摘要(Abstract)：

目的:探讨肿瘤坏死因子(TNF-β)G252A基因在子宫内膜异位症发病机制中作用。方法:80例经腹腔镜和病理确诊的内异症(Ems)患者为Ems组,80例同期需采用腹腔镜排除子宫内膜异位症的健康妇女为对照组,取两组妇女静脉血,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测TNF-βG252A基因的基因频率,酶联免疫(ELISA)法测定血清TNF-β的含量,分析子宫内膜异位症与TNFβG252A基因之间的关系。结果:Ems组血清TNF-β的含量明显高于对照组(P<0.05),且Ems组血清中TNF-β的含量与EMT的分期呈正相关(r=0.8532);Ems组的A/A基因型及G/A基因型频率均明显高于对照组,差异有统计学意义(P<0.05);A/A基因型患Ems的危险度是G/G基因型的3.5倍。结论:Ems患者血清中TNF-β的含量与EMT的分期呈正相关,A/A基因型是患Ems的危险基因型。
Objective: To investigate the role of TNF-β G252 A gene in pathogenesis of endometriosis( Ems). Methods: Eighty Ems patients diagnosed by laparoscopy and histopathologic examination served as Ems group and 80 healthy women served as control group. PCR-RFLP was used to detect gene frequency of TNF-β G252 A gene,and ELISA was applied to check the serum content of TNF-β.The relationship of Ems and TNF-β G252 A gene was analyzed. Result: The serum level of TNF-β in Ems group was higher than that in control group( P < 0. 05),and the serum level of TNF-β in Ems group was positively associated with EMT period. A/A and G/A genotypic frequencies in Ems group were significantly higher than those in control group. The risk of Ems of A/A genotype was 3. 5 times of G/G genotype. Conclusions: The serum level of TNF-β in Ems patients is positively associated with EMT period,and A/A genotype is the risk genotype of Ems.

关键词(KeyWords)： 子宫内膜异位症;基因,TNF-β G252A;基因频率;相关性;易感性;新生血管生成
endometriosis;gene,TNF-β G252A;gene frequency;relevance;susceptibility;angiogenesis

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 余鸿标;姚吉龙;
YU Hongbiao;YAO Jilong;Department of Gynecology,Luohu hospital of Shenzhen;Department of Gynecology,Maternal and Child Health Hospital of Shenzhen;

DOI: 10.19367/j.cnki.1000-2707.2015.03.016

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