贵州医科大学学报

2016, v.41;No.189(06) 649-652

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Calpain-1及Calpain-2在不同迁移能力肝癌细胞系中的表达
Expression of Calpain 1 and 2 in Liver Cancer Cells with Different Migration Abilities

王宁;陈腾祥;刘振华;王飞清;潘娅;
WANG Ning;CHEN Tengxiang;LIU Zhenhua;WANG Feiqing;PAN Ya;Department of Pharmaceutical Preparation,Guizhou Province Ostelolgical Hospital;Department of Physiology,Guizhou Medical University;Depatment of Liver and Gall Surgery,Guizhou Provincial People's Hospital;Clinical Laboratory,Guizhou Provincial Hospital of Traditional Chinese Medicine;

摘要(Abstract):

目的:研究钙激活中性蛋白酶蛋白-1(Calpain-1)及Calpain-2在不同迁移能力肝癌细胞系中的表达。方法:常规细胞培养高迁移能力的MHCC97-H、低迁移能力的MHCC97-L、无迁移能力的Hep G2的人肝癌细胞及正常人肝HL-7702细胞,采用western-bolt方法观察Calpain-1及Calpain-2在3株人肝癌细胞及正常肝细胞中的表达。结果:Calpain-1在正常肝细胞HL-7702、高迁移能力的MHCC97-H、低迁移能力的MHCC97-L及无迁移能力的Hep G2细胞中均有表达,表达量差异无统计学意义,P>0.05;与正常肝细胞HL-7702比较,高、低、迁移能力肝癌细胞系中Calpain-2不同程度表达,随着细胞迁移能力增强而成递增趋势,P<0.01或P<0.05。结论:Calpain-2随肝癌细胞迁移能力的增强表达增加,表明Calpain-2与肝癌细胞的侵袭转移可能有密切关系。
Objective: To investigate protein expression of Calpain-1 and Calpain-2 in liver cancer cell line with different migration ability. Methods: MHCC97-H( highly migratory liver cancer cells),MHCC97-L( lowly migratory liver cancer cells),Hep G2( liver cancer cells with no migration ability)and HL-7702( normal liver cells) were routinely cultured. Western-blot method was used to observe protein expression of Calpain-1 and Calpain-2 in the three human liver cancer cell lines and the normal liver cell. Results: There were protein expressions of Calpain-1 in HL-7702,MHCC97-H,MHCC97-L and Hep G2,and the difference was not statistically significant( P < 0. 05). Compared with normal liver cells HL-7702,Calpain-2 showed the different degrees of protein expression in high,low,migration ability of liver cancer cell line,and the protein expression of Calpain-2 significantly enhanced with increasing cell migration ability( P < 0. 01 or P < 0. 05). Conclusions: Calpain-2 shows an increasing protein expression with the enhancement of the migration ability of liver cancer cells,which indicates that calpain-2 may be closely related to the invasion and metastasis of liver cancer cells.

关键词(KeyWords): 癌,肝细胞;钙激活中性蛋白酶蛋白;迁移能力
carcinoma;hepatocellular;Calpain;migration ability

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅科研基金[黔科合LG字(2011)008];; 贵州省教育厅自然科学研究项目[黔教科(2008)022]

作者(Author): 王宁;陈腾祥;刘振华;王飞清;潘娅;
WANG Ning;CHEN Tengxiang;LIU Zhenhua;WANG Feiqing;PAN Ya;Department of Pharmaceutical Preparation,Guizhou Province Ostelolgical Hospital;Department of Physiology,Guizhou Medical University;Depatment of Liver and Gall Surgery,Guizhou Provincial People's Hospital;Clinical Laboratory,Guizhou Provincial Hospital of Traditional Chinese Medicine;

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DOI: 10.19367/j.cnki.1000-2707.2016.06.007

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