贵州医科大学学报

2016, v.41;No.186(03) 294-297+303

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饮用水有机提取物对L02细胞增殖及细胞周期的影响
The Influence on Cell Proliferation and Cell Cycle of L02 Cells Exposed to Organic Extracts from Drinking Water

岑延利;杨光红;桂晓玲;张爱华;李传美;
CEN Yanli;YANG Guanghong;GUI Xiaoling;ZHANG Aihua;LI Chuanmei;School of Public Health,Guizhou Medical University;

摘要(Abstract):

目的:探讨饮用水有机提取物对人正常肝细胞(L02)的细胞增殖及细胞周期的影响。方法:体外培养L02细胞,将其分别暴露于培养液(空白对照组)、0.1%DMSO(溶剂对照组)和0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/m L固相萃取法提取的饮用水有机提取物(染毒组)中24、48及72 h,采用MTT法观察L02细胞的增殖情况,流式细胞仪检测细胞周期的构成比。结果:(1)与溶剂对照组比较,各染毒组细胞活力随着有机提取物浓度的增加及时间的延长而降低,差异有统计学意义(P<0.05);(2)随染毒剂量的增加,细胞周期中G0/G1期细胞比例逐渐下降,除24 h、48 h 0.312 5 L/m L染毒组外,其余剂量染毒组G0/G1期细胞比例均明显低于溶剂对照组(P<0.05);S期细胞比例则随有机提取物浓度的增加而上升,除24、48及72 h 0.312 5 L/m L的染毒组外,其余染毒组中S期细胞比例的增加与对照组比较,差异有统计学意义(P<0.05);染毒48 h、72 h后,L02细胞中G0/G1期细胞比例低于24 h染毒组(P<0.05);S期细胞比例则高于24 h染毒组(P<0.05)。结论:在本研究条件下,饮用水有机提取物可以阻滞L02细胞于S期,抑制细胞的增殖,且呈时间、剂量依赖性。
Objective: To investigate the influence of organic extracts from drinking water on liver cell( L02) proliferation and cell cycle. Methods: The L02 cells was cultivated in vitro: blank control group( culture solution),solvent control group( 0. 1% DMSO); organic extracts group( The dose of pollutants groups were 0. 312 5,0. 625 0,1. 250 0,2. 500 0 and 5. 000 0 L / m L samples were extracted by solid phase extraction method). Each group was treated for 24 h,48 h and 72 h. Cell proliferation was measured by MTT assay. The constituent ratio of cell cycle was detected with flow cytometry.Results:( 1) Compared with solvent control group,cell vitality of L02 cells decreased gradually with the increasing of exposure time and concentration of organic extracts. The differences were statistically significant( P < 0. 05).( 2) The proportion of cells in G0 / G1 phase decreased gradually with the increasing concentration of organic extracts. Except for 0. 312 5 L / m L of pollutants group at 24 h and48 h,significant differences were found in other exposure groups( P < 0. 05). The proportion of cell in S phase increased gradually with the increasing concentration of organic extracts. Except for0. 312 5 L / m L at 24 h,48 h and 72 hr exposure,significant differences existed compared with solvent control group,differences were statistically significant( P < 0. 05). Compared with 24 h exposure group,exposed groups at 48 h- exposure and 72 h- exposure demonstrated significantly increase in G0 / G1 phase cell proportion and S phase cell proportion with increasing time( P < 0. 05). Conclusions: The results of the present paper showed that the organic extracts from drinking water can inhibit cell proliferation and change cell cycle of L02 cells.

关键词(KeyWords): 饮用水;有机提取物;肝细胞;细胞增殖;细胞周期
drinking water;organic extracts;hepatic cells;cell proliferation;cell cycle

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金[黔科合LH字(2014)7102];; 贵阳市科学技术计划项目[筑科合同(2013103)19号]

作者(Author): 岑延利;杨光红;桂晓玲;张爱华;李传美;
CEN Yanli;YANG Guanghong;GUI Xiaoling;ZHANG Aihua;LI Chuanmei;School of Public Health,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.03.013

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