孙广;李小多;张海龙;唐文凯;张云东;方文;
SUN Guang;LI Xiaoduo;ZHANG Hailong;TANG Wenkai;ZHANG Yundong;FANG Wen;Department of Clinical Laboratory,the People's Hospital of Anshun;College of Clinical Laboratory Sicence,Guizhou Medical University;

• 1:安顺市人民医院医学检验科
• 2:贵州医科大学医学检验学院

摘要(Abstract)：

目的:评估29种中药水提物和醇提物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抑菌活性,筛选抑菌活性最高的中药做进一步研究。方法:用K-B纸片法筛选出有抑菌活性的中药;用微量稀释法测定中药的最低抑菌浓度(MIC)和最低杀菌浓度(MBC);并用微量棋盘稀释法测定联合用药对MRSA的抑制作用。结果:K-B纸片法筛选结果显示,29种中药水提物仅黄柏有较小的抑菌圈,7种中药的醇提物有抑菌圈,其中黄柏的抑菌圈直径最大,均值为18.1 mm;金银花和紫花地丁抑菌圈不透明; 29种中药醇提物黄柏对MRSA的抑菌活性最强,其MIC的生药浓度为125 g/L,MBC的生药浓度为250 g/L;甘草与黄苓联合应用时,抗菌指数FIC <0.5。结论:所选中药醇提物对MRSA的抑菌活性强于水提物,黄柏醇提物最能有效抑制MRSA的生长;甘草与黄苓联合应用具有协同作用,对MRSA的抑菌活性最高。
Objective: To evaluate antibacterial activity of traditional Chinese medicine water extracts and ethanol extracts against methicillin-resistant staphylococcus aureu( MRSA) in vitro,screening the traditional Chinese medicine with the highest antibacterial activity for further study. Methods: The Chinese medicine with the powerful antibacterial activity against MRSA were selected by K-B paper dispersion; The minimal inhibitory concentration( MIC) and minimal bactericidal concentration( MBC) were determined by micro dilution method; The microdilution and checkerboard method were used to determine inhibitory effect of the Chinese medicine combination against MRSA. Results: Only water extracts of Golden Cypress had the smaller inhibition zone,ethanol extracts of 7 traditional Chinese medicine had inhibition zone, and the Golden Cypress had the biggest and transparent inhibition zone,18. 1 mm; Honeysuckle and Herba violae had the non-transparent inhibition zone. The results showed that the Golden Cypress ethanol extracts had the strongest antibacterial activity,the crude drug concentration of MIC was 125 g/L; that of MBC was 250 g/L. When Liquorice was combined with Radix Scutellariae,FIC index was < 0. 5. Conclusion: The ethanol extracts have stronger antibacterial activity than the water extracts to MRSA in vitro. The MIC and MBC of Golden Cypress ethanol extracts have minimum and thereby inhibit the growth of MRSA most effectively.According to the judgment standard of the FIC index,the antibacterial activity of Liquorice combined with Radix Scutellariae in vitro performances as synergistic effect against MRSA,and the antibacterial activity is the highest.

关键词(KeyWords)： 抗甲氧西林金黄色葡萄球菌;中药提取物;K-B纸片;最低抑菌浓度;最低杀菌浓度
methicillin-resistant Staphylococcus aureus(MRSA);TCM Extracts;K-B paper;MIC;MBC

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81560481);; 贵州省安顺市科技厅重点研发与推广项目[安市科工(2017)007号，安市科平(2017)01号]

作者(Authors): 孙广;李小多;张海龙;唐文凯;张云东;方文;
SUN Guang;LI Xiaoduo;ZHANG Hailong;TANG Wenkai;ZHANG Yundong;FANG Wen;Department of Clinical Laboratory,the People's Hospital of Anshun;College of Clinical Laboratory Sicence,Guizhou Medical University;

DOI: 10.19367/j.cnki.2096-8388.2020.10.011

参考文献(References)：

• [1]JEVONS,M. Methicillin resistance in staphylococci[J].Lancet,1963,281(7287):904-907.
• [2]张亚彬，王佳伟．对耐甲氧西林金黄色葡萄球菌耐药性及耐药基因分型的探讨[J]．当代医药论丛，2020,18(4):120-124.
• [3]CHAMBERS H F. The changing epidemiology of Staphylococcus aureus[J]. Emerge Infect Dis,2001,7(2):178-182.
• [4]时文艳．耐甲氧西林金黄色葡萄球菌SCC mec基因分型检测与耐药性研究[D]．长春:吉林大学，2015.
• [5]LEE A S,HUTTNER B,HARBARTH S. Control of methicillin-resistant staphylococcus aureus[J]. Infect Dis Clin North Am,2011,25:155-179.
• [6]张志军，曹海燕，等．医院感染金黄色葡萄球菌耐药表型与耐药基因研究[J]．中华医院感染学杂志，2015,25(9):1925-1926.
• [7] HADLER,JAMES L. Trends in invasive infection with Methicillin-Resistant staphylococcus aureus,connecticut,USA,2001-2010[J]. Emerging Infectious Diseases,2012,18(6):917-924.
• [8]袁文常．金黄色葡萄球菌适应性耐药及MRSA耐药调控机制的研究[D]．重庆:第三军医大学，2013.
• [9]杨海燕，张传美，吴金凤．七种中药对金黄色葡萄球菌的体外抑制试验[J]．动物医学进展，2007,28(8):48-51.
• [10]何顺志，徐文芬，黄敏，等．贵州中药资源种类、地理分布及开发利用现状[J]．贵州科学，2004,22(2):45-52.
• [11]宓伟，何深一．抗耐甲氧西林金黄色葡萄球菌的中药筛选[J]．中国生化药物杂志，2011,32(1):44-46.
• [12]李伟奇，刘湘新，李俊超，等．10种中药提取物对耐药金黄色葡萄球菌抑菌作用的研究[J]．动物医学进展，2010,31(9):41-46.
• [13]李敏敏，刘芳萍，马欣，等．黄连提取物和环丙沙星联用对鸡源沙门菌体外抑制作用的研究[J]．中国家禽，2016,38(2):14-17.
• [14]王爽，宋志香．耐甲氧西林金黄色葡萄球菌感染的临床特征、耐药性及治疗研究进展[J]．实用医院临床杂志，2009,6(4):137-139.
• [15]余琼．抗耐甲氧西林金黄色葡萄球菌(MRSA)天然产物的筛选及研究[D]．重庆:西南大学，2013.
• [16]万钧，李羲，贾坤茹，等．医院与社区获得性耐甲氧西林金黄色葡萄球菌耐药性的研究[J]．中华医院感染学杂志，2005,15(6):668-670.
• [17]何顺志．黔西北高原药用植物区系成分的初步研究[J]．贵州科学，1993,11(4):12.
• [18]何顺志，邹亚邦．贵州西南部分地区药用植物资源[J]．中国中药杂志，1994,19(7):392.
• [19]晏晨，张云东，王星慧，等．川黄柏果实中的化学成分及抗肿瘤活性[J]．天然产物研究与开发，2017,29(8):1270-1276,1438.
• [20]YAN C,ZHANG Y D,WANG X H,et al. Tirucallanetype triterpenoids from the fruits of Phellodendron chinense Schneid and their anti-cancer activities[J]. Fitoterapia,2016,113:132-138.
• [21]李辉标，陈慕媛，丘振文，等．复方黄柏液治疗溃疡性结肠炎的Meta分析[J]．中医药导报，2018,24(7):98-101.

文章评论(Comment)：