王艳炜,吴变稳,赵天资,郭亚楠,苗华为
WANG Yanwei,WU Bianwen,ZHAO Tianzi,GUO Yanan,MIAO Huawei

• 1:河北省中医院心内科
• 2:中国人民解放军联勤保障部队第九八零医院

摘要(Abstract)：

目的:探讨丹夏降压颗粒对自发性高血压大鼠(SHR)收缩压及左心室肥厚(LVH)的影响。方法:16只12周龄SHR大鼠随机均分为模型组与中药组,另选8只同周龄Wistar大鼠作为对照组,中药组每天给予丹夏降压颗粒溶液灌胃,其余2组给予同等剂量蒸馏水处理,连续给药8周;分别于给药前、给药第4周及第8周时测量3组大鼠收缩压及心率;于第8周灌胃结束时,麻醉处死3组大鼠取左心室,检测心脏指数(HWI)及左心室指数(LVMI),采用Western blot检测心肌细胞心肌营养素-1(CT-1)蛋白含量。结果:与对照组比较,模型组和给药组大鼠收缩压显著升高(P<0.01);给药后中药组大鼠收缩压较给药前及同时段模型组显著降低,且治疗第8周显著低于治疗第4周(P<0.01);给药8周时,模型组和给药组大鼠的HWI和LVMI均显著高于对照组大鼠(P<0.01),中药组大鼠HWI和LVMI较模型组显著下降(P<0.01);大鼠心肌细胞CT-1蛋白含量比较,模型组和给药组均显著高于对照组大鼠(P<0.01),中药组较模型组显著下降(P<0.01)。结论:丹夏降压颗粒能有效降低收缩压、改善左心室肥厚,其机制可能与其减少心肌CT-1的表达有关。
Objective: To investigate the effects of Danxia granules on the systolic blood pressure,the left ventricular hypertrophy(LVH) and the expression of cardiotrophin-1 of spontaneously hypertensive rats(SHR).Method: 16 twelve-week-old SHR were randomly divided into the model group(n=8)and Danxia granules group(n=8). Age-matched Wistar rats served as the normal blood pressure control group(n=8). The Danxia granules group was fed with Danxia granules and the other two groups were fed with distilled water. The experiment lasted for 8 weeks. Systolic blood pressure and heart rate were measured before, the 4 th week and 8 th week after the experiment. After 8-week treatment, heart weight index(HWI)and left ventricular weight index(LVWI)were measured and cardiotrophin-1(CT-1)was examined by Western blot. Results: The systolic blood pressure, HWI and LVWI all decreased significantly in Danxia granules group compared with the model group. Compared with the control group, the expression level of CT-1 in myocardial tissues were obviously higher in the model group and Danxia granules group(P<0.05), and the expression level of CT-1 decreased in Danxia granules group compared with the model group(P<0.05).Conclusion: Danxia granules showed a certain effect on the reduction of the systolic blood pressure and the expression level of cardiac partial CT-1.It reversed the LVH.

关键词(KeyWords)： 丹夏降压颗粒;心脏指数;左心室指数;心肌营养素-1;自发性高血压大鼠
Danxia granules;heart weight index;left ventricular weight index;cardiotrophin-1;spontaneously hypertensive rats(SHR)

Abstract:

Keywords:

基金项目(Foundation): 河北省中医药管理局课题(2019054)

作者(Author): 王艳炜,吴变稳,赵天资,郭亚楠,苗华为
WANG Yanwei,WU Bianwen,ZHAO Tianzi,GUO Yanan,MIAO Huawei

DOI: 10.19367/j.cnki.1000-2707.2020.01.016

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