齐晓岚,张雪玲,官志忠
QI Xiaolan,ZHANG Xueling,GUAN Zhizhong (Department of Pathology

• 1:贵阳医学院病理学教研室及贵阳医学院分子生物学重点实验室

摘要(Abstract)：

目的:研究老年性痴呆发病中α7尼古丁受体(nAChR)与ERK MAPK信号转导通路之间的关系。方法:用MEK阻断剂U0126阻断SH-SY5Y细胞ERK蛋白的活化及表达,实时荧光定量PCR(Real-time PCR)及Western blotting方法测定α7 nAChR mRNA及蛋白表达水平。结果:细胞经U0126处理后p-MEK和p-ERK1/2的水平明显降低,提示MEK和ERK 1/2的磷酸化被阻断,同时U0126处理组α7 nAChR mRNA及蛋白的表达量降低。结论:U0126能抑制SH-SY5Y细胞ERK MAPK信号转导通路,ERK MAPK信号转导通路的活化可能与神经细胞α7尼古丁受体的正常分泌及胆碱能信号传递密切相关。
Objective:To study the relationship between α7 nicotinic acetylcholine receptor(nAChR) and extracellular-signal regulated kinase(ERK) signal transduction pathway in patients with Alzheimer's disease.Methods:SH-SY5Y cells were exposed to U-0126,a blocker of MEK protein,to block the activation and expression of ERK protein.The levels of α7 nAChR mRNA and protein were measured by real-time PCR and western blotting respectively.Results:The expression of p-MEK and p-ERK1/2 in SH-SY5Y cells remarkablly decreased after the treatment of U0126,which indicated that the phosphorylation of MEK and ERK 1/2 were blocked.The expression of α7 nAChR mRNA and protein decreased in U0126 treated group in the same time.Conclusions:ERK MAPK signal transduction pathway can be blocked by U-0126 in SH-SY5Y cells.ERK MAPK signal transduction pathway may correlate with the secretion of α7 nAChR and cholinergic signal transduction.

关键词(KeyWords)： 细胞外信号调节激酶;α7神经型尼古丁受体;阿尔茨海默病;SH-SY5Y神经母细胞瘤细胞
extracellular-signal regulated kinase;α7 nicotinic acetylcholine receptor;Alzheimer disease;SH-SY5Y neuroblastoma cell

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目:30960123;; 贵州省科技厅基金项目:黔科合2010[3140]

作者(Author): 齐晓岚,张雪玲,官志忠
QI Xiaolan,ZHANG Xueling,GUAN Zhizhong (Department of Pathology

DOI: 10.19367/j.cnki.1000-2707.2011.04.004

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