陈艳,毛克强
Chen Yan, et al Department of Parasitology

• 1: 贵阳医学院寄生虫学教研室
• 2:贵阳医学院寄生虫学教研室

摘要(Abstract)：

检测宿主体内的CAg可以早期诊断寄生虫感染,鉴于应用PcAb-ABC-Dot-ELISA检测弓形虫CAg国内尚未见报道,作者用生物素化抗弓形虫PcAb为探针,检测了15份人工感染弓形虫的兔血清,结果15份血清的CAg均呈阳性反应。用35份正常兔血清,10份自然感染球虫的兔血清及血吸虫、华校睾吸虫感染的兔血清各5份作对照,除一份正常兔血清及一份血吸虫感染的兔血清呈阳性反应外,其余均为阴性。结果表明,该法有较好的特异性及重复性。
Although the T. gondii circulating antigen was successfully detected with McAb-ELISA, the utilization of PcAb-ABC-Dot-ELISA jn detecting it has not been reported yet. Using biotin labelled PcAb against T. gondii as a probe, an experiment was conducted to detect the circulating antigen in sera of infected rabbits with PcAb-ABC-Dot-ELISA. The results were as follows. All the sera from 15 infected rabbits showed positive reactions on the 13th day after infection whereas only one of the 35 normal controls gave a posititive reaction. 10 sera from the rabbbit Infected with Eimeria sp, 5 with C. sinensis and 5 with S. japunicum weretested. Among them, only one of those infected with S. japonicum show ed a postive reaction. Our studies showed that the PcAb-ABC-Dot-ELISA is a sensitive and specific method and it's reproducibility is good for detecting CAg of T. gondii in the sera.

关键词(KeyWords)： 动物弓形体病;抗原;酶联免疫吸附测定
toxoplasmosis, animal; antigen; enzyme-linked immunosorbent assay

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陈艳,毛克强
Chen Yan, et al Department of Parasitology

DOI: 10.19367/j.cnki.1000-2707.1991.04.013

参考文献(References)：

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• 2． 黎世涛,等.ABC一抗CSA系统作为检测日本血吸虫病循环抗原探针建立和初步研究,免疫学杂志 1988;4(1) :40～43．
• 3． 张一伟,等.应用单克隆抗体Dot- ELISA检测血吸虫循环抗原的初步研究.四川寄生虫病防治杂志 1988;16(1) :9-11．
• 4． 胡孝素,等.用McAb检测患者血清循环抗原渗断黑热病的研究.华西医科大学学报1986;17(1) :1-4．
• 5． 傅翠娥,等.感染弓形虫家兔血清循环抗原、免疫复合物及抗体的消长情况规律实验研究,浙江省医学科学院院报 1987;(38) :78-81．

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