朱海涛,董琼珠,贾户亮,钦伦秀
ZHU Haitao1,DONG Qiongzhu2,JIA huliang2,QIN Lunxiu2(1.Department of Hepatobiliary Surgery

• 1:贵阳医学院附院肝胆外科
• 2:复旦大学附属中山医院

摘要(Abstract)：

目的:探讨甲型肝炎病毒感染患者血清中miR-122表达水平与健康人及乙型肝炎病毒感染患者的差异。方法:24例甲型肝炎患者、22例乙型肝炎患者及25例健康人血清分别抽提总RNA,用实时定量PCR分析其中miR-122的表达并比较差异。结果:甲型肝炎患者血清中miR-122水平与健康人比较无显著差异(P>0.05),乙型肝炎患者血清中miR-122水平较甲型肝炎患者及健康人均显著升高(P<0.05)。结论:miR-122在甲型肝炎患者血清中较健康人无显著差异,而乙型肝炎患者血清中miR-122表达高于正常水平,提示miR-122可能不适合作为甲型肝炎相关肝病的标记物。
Objective: To compare the serum level of miR-122 in hepatitis A virus-infected patients with both that of healthy people and hepatitis B virus-infected patients.Methods: Serum total RNA of 24 hepatitis A virus-infected patients,22 hepatitis B virus-infected patients and 25 healthy people were extracted,and their expression levels of miR-122 were detected with quantitative real-time polymerase chain reaction analyses and compared.Results: No statistical difference was found in serum levels of miR-122 between hepatitis A virus-infected patients and healthy people(P>0.05).Serum level of miR-122 in hepatitis B virus-infected patients was higher than that of hepatitis A virus-infected patients and healthy people(P<0.05).Conclusions: The results indicate the serum miR-122 level may not be used as biomarker for hepatitis A virus-related hepatic disease.

关键词(KeyWords)： 微RNAs;肝炎,甲型;肝炎,乙型;聚合酶链反应;血清学
microRNAs;hepatitis A;hepatitis B;polymerase chain reaction;serology

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 朱海涛,董琼珠,贾户亮,钦伦秀
ZHU Haitao1,DONG Qiongzhu2,JIA huliang2,QIN Lunxiu2(1.Department of Hepatobiliary Surgery

DOI: 10.19367/j.cnki.1000-2707.2012.03.016

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