赵燕,蔡美英,王霞,魏大鹏
ZHAO Yan1,CAI Meiying2,WANG Xia2,WEI Dapeng2(1.Department of Laboratory Science

• 1:成都医学院检验系
• 2:四川大学基础医学与法医学院免疫学教研室

摘要(Abstract)：

目的:探讨口服Ⅱ型胶原(CⅡ)对大鼠佐剂型关节炎(AA)的治疗作用。方法:建立大鼠AA动物模型,观察口服高、中、低剂量的CⅡ后大鼠关节病变情况,流式细胞仪检测大鼠外周血T细胞亚群的变化,原位杂交法检测大鼠脾脏和派氏淋巴结(Peyerpatchespp)中转化生长因子β(TGF-β)、IL-12水平的变化。结果:口服高、中、低剂量的Ⅱ型胶原均可减轻关节病变的发生,高剂量组作用最显著,大鼠外周血CD4+T细胞和CD8+T细胞水平与模型组比较均有显著性差异,脾脏和pp淋巴结TGF-β水平较模型组升高,IL-12无明显改变,pp淋巴结TGF-β阳性细胞数高于脾脏。结论:TGF-β是口服耐受抑制AA的重要细胞因子,肠道黏膜免疫系统在口服耐受的诱导中可能发挥重要作用。
Objective:To investigate the effects of oral administration of collagen(CⅡ)on TGF-β and IL-12 in rats with adjuvant arthritis(AA).Methods:Rat AA model was established by immunization with Freund's adjuvant,and received different doses of CⅡ orally during a 2-week period afterwards.Incidence and severity of arthritis were assessed by calculation of mean arthritis index.T cell subsets in peripheral blood were assayed by immunofluorescence technique and concentrations of TGF-β,IL-12 in spleen and Peyer patches(pp)of rats were determined with in situ hybridization kit separately.Results:CⅡ at different doses could ameliorate the adjuvant induced arthritis,especially at high dose.Compared with control group,T cell subset CD4 decreased while T cell subset CD8 increased significantly when treated with high dose of CⅡ,and TGF-β concentration also increased significantly,especially in pp.No change of IL-12 content was found.There were more TGF-β positive cells in pp than in spleen.Conclusions:TGF-β is one of the important inhibitory cytokines to AA with cell tolerance,and the immune system in intestinal mucosa may play an important role in suppression of AA by oral tolerence.

关键词(KeyWords)： 关节炎,实验性;转化生长因子β;胶原Ⅱ型;大鼠,Wister;口服耐受
arthritis,experimental;transforming growth factor beta;collagen type Ⅱ;rats,Wister;oral tolerance

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 赵燕,蔡美英,王霞,魏大鹏
ZHAO Yan1,CAI Meiying2,WANG Xia2,WEI Dapeng2(1.Department of Laboratory Science

DOI: 10.19367/j.cnki.1000-2707.2010.01.016

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