闵亚丽,蓝天座
MIN Ya-li,LAN Tian-zuo(Department of Nephrology

• 1:贵阳市第一人民医院肾内科
• 2:贵阳市第一人民医院肾内科 贵州贵阳550003
• 3:贵州贵阳550003

摘要(Abstract)：

目的:对肾脏结缔组织生长因子(CTGF)、α平滑肌肌动蛋白(α-SMA)在单侧输尿管结扎致大鼠肾间质纤维化模型中的表达进行动态观察,以探讨CTGF在肾间质纤维化病程中的作用及意义。方法:采用单侧输尿管结扎术(UUO)致大鼠肾间质纤维化模型,分别于术后第1、3、5、7、9、12天处死大鼠,HE、M asson染色观察病理改变并进行小管间质损伤指数评分,免疫组织化学方法观察各组大鼠CTGF、α-SMA的表达。结果:第1~9天大鼠肾间质纤维化病理改变逐渐明显,CTGF、α-SMA表达逐渐增多(各组间比较,P<0.05);CTGF与肾小管间质损伤指数、α-SMA为正相关关系。结论:CTGF在肾小管间质纤维化的病程进展中起重要的作用,并可作为评价肾纤维化病变程度的良好指标及防治的新的靶目标。
Objective: To continuously observe the expression of CTGF and α-SMA in renal tubulointerstitial fibrosis of rats,and to investigate the significance of fibrogenic effects of CTGF.Methods: Unilateral ureteral obstruction(UUO) was adopted to produce rat models of renal tubulointerstitial fibrosis,and the rats were executed on the 1~(st),3~(rd),5~(th),7~(th),9~(th) and 12~(th) days post-operation respectively.The expression of CTGF,α-SMA in the obstructed kidney were measured by immunohistochemistry.At the same time,the degree of tubulointerstitial damage was scored.Results: The levels of CTGF,α-SMA increased gradually from the first day to the 9th day(P<0.01) and paralleled the degree of tubulointerstitial damage.CTGF had positive correlation with the indexes of tubulointerstitial damage and α-SMA.Conclusions: CTGF may play an important role in the development of tubulointerstitial fibrosis and become a new cure target for the prevention of the disease.

关键词(KeyWords)： 生长物质;结缔组织;肌动蛋白类;肾功能衰竭,慢性;纤维化;大鼠
growth substances;connective tissue;actins;kidney failure,chronic;fibrosis; rats

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 闵亚丽,蓝天座
MIN Ya-li,LAN Tian-zuo(Department of Nephrology

DOI: 10.19367/j.cnki.1000-2707.2006.03.017

参考文献(References)：

• [1]Eddy AA.Molecu lar basis of renal fibrosis[J].Ped iatrNephrol,2000,15(3~4):290-295.
• [2]Radford G,Donad lo J.Pred icting renal outcom e in IgAnephropathy[J].J Am Soc Nephrol,1997,8(2):199-207.
• [3]B radham DM,Igarashl A,Potter RL,et al.Connectivetissue growth factor:A cydte ine-rich m itogen secretedby hum an vascu lar endothelial cells is related to the SRC-induced imm ed iate early gene product CEF-10[J].JCell B iol,1991,114:1285-1294.
• [4]Ito Y,Bende R J,Oem ar BS.Expression of connective tis-sue growth factor in hum an renal fibrosis[J].K idney Int,1998(53):853-857.
• [5]黄海长,李惊子,王海燕.结缔组织生长因子诱导成纤维细胞转化为成肌纤维细胞[J].科学通报,2000,47(1):37-40.
• [6]杨敏,黄海长,李惊子,等.结缔组织生长因子协同转化生长因子β1的促肾纤维化效应[J].中华医学杂志,2004,84(7):569-573.

文章评论(Comment)：