王丽娜,吴晓琳
WANG Lina,WU Xiaolin

• 1:西安市第九医院神经内科

摘要(Abstract)：

目的:探讨地黄饮子汤对急性脑缺血再灌注大鼠行为学及抗氧化能力的影响。方法:选取清洁级Wistar大鼠60只,随机分为对照组、模型组、西药组(尼莫地平)和实验组(地黄饮子汤),各15只;模型组、西药组及实验组大鼠,制作急性脑缺血再灌注大鼠模型,对照组大鼠只做颈部手术但不造模;造模后对照组及模型组每日灌胃生理盐水10 m L/kg,西药组每日灌胃尼莫地平12.5 mg/kg,实验组每日灌胃地黄饮子汤40 m L/kg,每日1次,连续灌胃3周;干预结束后第1、2、3周进行行为学Longa评分和Berderson评分,并于造模后第3周眼眶取血及脑组织,分别检测脑组织和血清,超氧化物歧化酶(SOD)、丙二醛(MDA)、可见光分光光度法检测过氧化氢酶(CAT)、比色法检测谷胱甘肽过氧化酶(GSH-Px)水平及活性。结果:与模型组比较,实验组的第1、2、3周和西药组的第3周时Longa评分、Berderson评分明显降低,差异有统计学意义(P<0.05);与西药组比较,实验组的第1、2、3周时Longa评分、Berderson评分明显降低,差异有统计学意义(P<0.05);与西药组比较,实验组大鼠血清中SOD、MDA、CAT水平及GSH-Px活性比较,差异有统计学意义(P<0.05);与西药组比较,实验组的脑组织中SOD、MDA、CAT、GSH-Px差异有统计学意义(P<0.05)。结论:地黄饮子汤能有效治疗缺血性脑中风,其机制可能是增强脑组织及血清的抗氧化能力,促进神经功能修复,改善行为能力,修复受损的神经元。
Objective: To explore the effects of rehmannia Yinzi decoction on the praxiology and antioxidant capacity of rats with acute cerebral ischemia reperfusion. Methods: 60 clean Wistar rats were selected and randomly divided into the control group,the model group,the western medicine group( nimodipine),and the experimental group( radix rehmanniae Yinzi decoction),15 in each group.Rat models of cerebral ischemia reperfusion were made in the model group,the western medicine group,and the experimental group. After modeling,gastric lavage with physiological saline( 10 ml/kg) was carried out daily in the control group and model group,lavage with nimodipine of 12. 5 mg/kg daily in the western medicine group,lavage of rehmannia Yinzi decoction of 40 ml/kg daily in the experimental group,All the gastric lavages were made once a day,lasting for 3 weeks. After intervention Longa and Berderson scores were used to detect the praxiology,brain tissue and serum,Xanthine oxidase method to detect SOD MDA,visible spectrophotometry to detect the CAT,colorimetry to detect the GSH-Px level. Results: Longa and Berderson scores in the experimental group in the 1 ~(st),2 ~(nd) and 3 ~(rd) weeks and the western medicine group in the 3 rd week were significantly lower than those in the model group,and the difference was statistically significant( P < 0. 05); Longa and Berderson scores were significantly lower in the experimental group in the 1 ~(st),2 ~(nd) and 3 ~(rd) weeks than those in the western medicine group,and the difference was statistically significant( P < 0. 05). The activities of SOD,MDA,CAT and GSH-Px in rat serum of the experimental group were quite different from those of the western medicine group,and the difference was statistically significant( P < 0. 05). There was significant difference between the western medicine group and experimental group in SOD,MDA,CAT,and GSH-Px in brain tissues,and it was statistically significant( P < 0. 05). Conclusion: Rehmannia Yinzi decoction can enhance brain tissue and serum antioxidant capacity,promote restoration of neurologic function,improve the capacity and repair damaged neurons.

关键词(KeyWords)： 地黄饮子汤;脑缺血;再灌注;行为学;抗氧化
Rehmannia Yinzi decoction;cerebral ischemia;reperfusion;praxiology;antioxidant

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 王丽娜,吴晓琳
WANG Lina,WU Xiaolin

DOI: 10.19367/j.cnki.1000-2707.2017.10.009

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