何燕;赵孝梅;左娅;张婷;邓婕;吴昌学;禹文峰;官志忠;
HE Yan;ZHAO Xiaomei;ZUO Ya;ZHANG Ting;DENG Jie;WU Changxue;YU Wenfeng;GUAN Zhizhong;The Key Laboratory of Molecular Biology,Guiyang Medical College;

• 1:贵阳医学院分子生物学重点实验室

摘要(Abstract)：

目的:了解贵州省3个少数民族(布依族、水族、苗族)群体人类白细胞抗原-G(HLA-G)基因14 bp插入/缺失多态性的分布规律及其与遗传背景之间的关系。方法:采用PCR扩增、电泳及测序的方法对来自贵州地区的布依族、水族、苗族共344例健康个体DNA进行HLA-G基因14 bp插入/缺失多态性检测,并与文献报道的仡佬族、壮族等13个民族群体的HLA-G基因14 bp插入/缺失多态性分布数据进行对比。结果:布依族、水族和苗族人群的+14 bp等位基因频率分别为23.7%、26.0%和38.1%,+14 bp/+14 bp基因型频率分别为9.40%、10.4%和15.9%,14 bp插入频率比较示布依族和苗族差异有统计学意义(χ2=11.345,P=0.001),苗族与水族差异有统计学意义(χ2=6.125,P=0.009)而布依族和水族间比较差异无统计学意义(P>0.017);与其他人群数据比较,苗族群体的14 bp插入/缺失分布与其他人群相似,而作为壮侗语族的布依族、水族则有自己独特的14 bp插入/缺失分布规律。结论:布依族、水族人群的14 bp插入/缺失分布相似,但是与苗族人群的分布存在一定的差异。
Objective: To investigate the distribution of HLA-G gene 14 bp insertion /deletion polymorphism in Buyi, Shui and Gelao populations and their relationship with genetic background.Methods: PCR amplification,agarose gel electrophoresis and sequencing were used to explore the distribution of 14 bp insertion/deletion in HLA-G gene in 344 healthy people of these three ethnic groups,and the data were compared with that of 13 ethnic groups such as Gelao,Zuang reported in the literature. Results: The frequencies of + 14 bp in Buyi,Shui and Miao were 23. 7%,26. 0% and 38. 1%respectively. The genotype frequencies of + 14 bp/+ 14 bp in Buyi,Shui and Miao were 9. 40%,10. 4% and 15. 9% respectively. The differences of 14 bp insertion frequencies between Buyi and Miao,between Miao and Shui were statistically significant( P < 0. 05),while no significant difference was found between Buyi and Shui( P > 0. 05). When compared with other groups,the distribution characteristic of + 14 bp insertion/deletion in Miao was similar to them,but Buyi and Shui showed significance difference from others. Conclusion: The distribution characteristics of + 14 bp insertion/deletion in Buyi is similar to Shui,but they are different from that of Miao.

关键词(KeyWords)： 基因,HLA-G;14 bp插入/缺失多态;布依族;水族;苗族;贵州
gene,HLA-G;+ 14 bp insertion/deletion polymorphism;Buyi people;Shui people;Miao people;Guizhou

Abstract:

Keywords:

基金项目(Foundation): 国家“十二·五”科技支撑计划项目(No:2013BAI05B03);; 贵州省科技厅社会发展攻关项目[No:SY(2012)3135]

作者(Authors): 何燕;赵孝梅;左娅;张婷;邓婕;吴昌学;禹文峰;官志忠;
HE Yan;ZHAO Xiaomei;ZUO Ya;ZHANG Ting;DENG Jie;WU Changxue;YU Wenfeng;GUAN Zhizhong;The Key Laboratory of Molecular Biology,Guiyang Medical College;

DOI: 10.19367/j.cnki.1000-2707.2015.03.001

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