吕心瑞,潘娅,李清春,董宇华,蒋乃昌
LV Xin-rui,PAN Ya,LI Qing-chun,DONG Yu-hua,JIANG Nai-chang(Department of Physiology

• 1:贵阳医学院生理学教研室
• 2:贵阳医学院生理学教研室
• 3:贵阳医学院生理学教研室
• 4:贵阳医学院生理学教研室

摘要(Abstract)：

目的:研究神经生长因子对拟阿尔茨海默病(A lzhe im er's D isease,AD)模型大鼠海马及皮层中神经原纤维缠结(NFT)的影响。方法:采用冈田酸(Okadaic Ac id,OA)海马CA1区微量多次注射建立拟AD大鼠模型,侧脑室注射神经生长因子(nerve growth factor,NGF)进行预处理,观察大鼠行为学变化,B ielschowsky染色观察海马及皮层神经原纤维缠结(neurobrillary tangles,NFT)。结果:OA注射后,大鼠出现认知能力、学习记忆能力减退,海马及皮层出现较多NFT;注射NGF预处理后,上述症状改善,海马及皮层NFT数量减少。结论:NGF可显著改善拟AD大鼠模型学习记忆能力,表明NGF可抑制NFT的形成,改善AD病的症状。
Objective: To investigate the effect of nerve growth factor(NGF) on neurofibrillary tangles(NFT) in hippocampal and cortex region of Alzheimer's disease(AD)-like model rats.Methods: Okadaic acid(OA) was injected into hippocamal CA1 region of rats with microdosis repeatedly to establish Alzheimer's disease-like model.NGF was injected into lateral cerebral ventricle as pretreatment.The behavior change of rats was observed.NFT in hippocampus and cortex was observed on Bielschowsky stained sections.Results: After injection of OA,the learning impairment and the memory deficits of rats was shown,and more NFT were appeared in hippocamal and cortex.After NGF pretreatment,symptoms mentioned above were remitted,and NFT amounts were reduced in hippocamal and cortex.Conclusions: NGF can impove the learning and memory capability of the model rats,inhibit the formation of NFT,and remit AD symptoms.

关键词(KeyWords)： 阿尔茨海默病;神经生长因子;神经原纤维缠结;大鼠
Alzheimer's disease;nerve growth factor;neurofibrillary tangles

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技教育人才省长专业基金资助项目(S2001-3)

作者(Author): 吕心瑞,潘娅,李清春,董宇华,蒋乃昌
LV Xin-rui,PAN Ya,LI Qing-chun,DONG Yu-hua,JIANG Nai-chang(Department of Physiology

DOI: 10.19367/j.cnki.1000-2707.2006.03.005

参考文献(References)：

• [1]Saido TC.Towards presymptomm atic d iagnosis,preventionand tream ent of A lzhe im er's d isease[J].R inshosh inke igaku,2004,44(11):824-826.
• [2]Barbara A,R icarda H,Josef K.Retinoic ac id potentiatedthe protective effect of NGF aga-inst staurosporine-inducedapoptosis in cu ltured ch ick neurons by increasing the trkApro-te in expression.J Neurosc i Res,2000(60):767-778.
• [3]周宁,杜冠华.Tau蛋白在A lzhm er’s病中的作用研究进展[J].中国药理学通报,2004,20(10):1085-1089.
• [4]Saleh iA,Delcroix JD,Swaab DG.A lzhe im er's d isease andNGF signaling[J].Neural Transm,2004,111(3):323-345.
• [5]HumpelC,W e is C.Nerve growth factor and cholinergicCNS neurons stud ied in organotyp ic brain slices:Imp lica-tion in A lzhe im er’d isease J Neural Transm Supp l,2002,(62):253-263.
• [6]Lad SP,Neek KE,Mufson EJ,et al.Nerve growth fac-tor:structure function and therapeutic imp lications forA lzhe im er's d isease[J].Curr D rug Target CNS Neurol D is-ord,2003,2(5):315-324.
• [7]Mufson EJ,G insberg SD,Ikonomovic MD,et al.Hum ancholinergic basal forebrain:Che moanatomy and neurologicdysfunction[J].J Chem Neukroanat,2003,26(4):233-242.

文章评论(Comment)：