贵州医科大学学报

2007, No.126(03) 221-224

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应用蛋白质组学技术分析肝癌细胞蛋白质的表达
A Comparative Proteome Analysis of Hepatoma Cell Cytosol

李兴;潘卫;邱峰;邱宗荫;
LI Xing1,PAN Wei1,QIU Feng2,QIU Zong-yin2(1.The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Pharmacology,Chongqing University of Medical Sciences,Chongqing 400016,China)

摘要(Abstract):

目的:细胞质在细胞内的代谢过程、信号转导等功能中起着重要的作用,以体外培养的人体肝癌细胞和正常肝细胞为研究对象,运用蛋白质组学的研究方法,对比分析肝癌细胞与正常肝细胞细胞质蛋白质的差异表达情况,为肿瘤发生机制的阐释提供信息。方法:体外培养的细胞(包括肝癌细胞、正常肝细胞)以超离心分离细胞质,双向电泳分离细胞质蛋白质,图像分析肝癌细胞与正常肝细胞的差异表达蛋白质,MALDI-TOF-MS鉴定差异蛋白质。结果:在肝癌细胞与正常肝细胞的对比分析中筛选出10个差异表达蛋白质,这些差异蛋白质涉及到细胞的能量代谢、蛋白质合成、调亡调控等方面。结论:细胞癌变后细胞质中蛋白质的表达发生了许多改变,可为肿瘤发生机制的阐释提供有价值的信息。
Objective:To compare the difference of cytosol protein expression between hepatoma cells and normal hepatocytes,and so as to provide contribution to the explanation of tumorigenesis and action mechanism of epirubicin.Methods: Cultured cells(including hepatoma cells and normal hepatocyte) were subjected to centrifugation for the fractionation of cytosol,and the cytosol proteins were separated by using two dimensional gel electrophoresis.The expression-altered protein spots were detected with imaging analysis,and the proteins were identified with MALDI-TOF-MS.Results: 10 expression-altered proteins were identified between the hepatoma cells and normal hepatocytes,and were shown to be involved in energy generation,protein biosynthesis,and apoptosis regulation of the cells.Conclusions: There occur many changes of cytosol protein expression in hepatoma cells after tumorigenesis,and the results of this study are helpful for us to understand tumorigenesis to some extent.

关键词(KeyWords): 肝肿瘤;肿瘤细胞,培养的;蛋白质组;细胞质
liver neoplasms;tumor cells,cultured;proteome;cytoplasm

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基金项目(Foundation): 国家自然科学基金资助项目(30476021);; 教育部博士点基金资助项目(教特发中心函[2004]166号)

作者(Author): 李兴;潘卫;邱峰;邱宗荫;
LI Xing1,PAN Wei1,QIU Feng2,QIU Zong-yin2(1.The Affiliated Hospital of Guiyang Medical College,Guiyang 550004,China;2.Department of Pharmacology,Chongqing University of Medical Sciences,Chongqing 400016,China)

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DOI: 10.19367/j.cnki.1000-2707.2007.03.002

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