杨文秀,李品浩,陈琴,裴媛媛
YANG Wenxiu,LI Pinhao,CHEN Qin,PEI Yuanyuan

• 1:贵州医科大学病理学教研室

摘要(Abstract)：

目的:观察白介素-6(IL-6)对伯基特淋巴瘤(BL)Raji细胞和弥漫大B细胞淋巴瘤(DLBCL)OCILY8细胞生长的影响,探讨信号转导子与转录活化-3(STAT3)及基质金属蛋白酶组织抑制因子-1(TIMP-1)分子在BL和DLBCL中发生发展的作用及其相互关系。方法:用不同浓度的IL-6(0、50、100、150及200μg/L)分别培养Raji细胞和OCI-LY8细胞24 h、48 h及72 h(分别为对照组和不同浓度IL-6试验组),用MTT法检查2株细胞生长情况,用RT-q PCR检测培养48 h时2株细胞STAT3、TIMP-1的m RNA表达,用Western blot检测100μg/L IL-6培养48 h时Raji细胞的STAT3、p-STAT3及TIMP-1蛋白的表达,流式细胞技术检测100μg/L IL-6培养48 h时2株细胞的细胞周期变化。结果:与相应的对照组比较,不同浓度IL-6试验组2株细胞在A490nm处OD值明显降低,呈现药物浓度依赖关系(P<0.01);2株细胞内的STAT3,TIMP-1的m RNA表达明显升高(P<0.05),呈现出药物浓度依赖关系(P<0.05),2株细胞内TIMP-1与STAT3的m RNA表达呈正相关关系(r=0.982,P=0.018);IL-6组Raji细胞中p-STAT3、STAT3和TIMP-1蛋白表达增高;IL-6组Raji和OCI-LY8细胞的G1期细胞都明显减少、S期细胞明显增多(P<0.05),G2-M期的Raji细胞无明显变化而OCI-LY8细胞则明显增多(P=0.037)。结论:IL-6对Raji细胞和OCI-ly8细胞生长有明显作用,STAT3活化及其下游靶基因TIMP-1的上调表达可能是IL-6影响2株细胞生长的重要分子机制。
Objective: To observe the effects of IL-6(interleukin-6) on the growth of Burkitt lymphoma( BL) cell line Raji and Diffuse large B cell lymphoma( DLBCL) cell line OCI-LY8,and to investigate their effect on cellular growth and the relationship between changes of STAT3( single transducer and transcription activater-3) and TIMP-1( tissue inhibitors of metalloproteinase-1) expression.Methods: Raji and OCI-LY8 cells were cultured by varied concentration of IL-6( 0 g / L,50 g / L,100 g / L,150 g / L and 200 g / L) for 24 h,48 h and 72 h. Viability of 2 strains of cells was measured by MTT. The m RNA expressions of STAT3 and TIMP-1 of 2 strains of cells were detected by RT-PCR,and p-STAT3,STAT3 and TIMP-1 protein expression of Raji cell cultivated by 100 g / L IL-6 for 48 h were detected by Western blotting. Cell cycle was examined by flow cytometry. Results: Comparing with control group,OD value at 490 nm decreased the two strain of cells treated by varied concentration of IL-6. It was presented concentration-dependence relationship( P < 0. 01); m RNA expressions of STAT3 and TIMP-1 in both cells exhibited positive correlation( r = 0. 982,P = 0. 018); protein expression of p-STAT3,STAT3 and TIMP-1 of Raji cells in IL-6 groups increased. Cells were distinctly reduced at phase G1 and increased at phase S in two types of cell of IL-6( P < 0. 05). The Raji cell at phase G2-M showed no significant change while OCI-LY8 cells increased significantly( P = 0. 037).Conclusions: IL-6 has an obvious effect on the growth of Raji and OCI-LY8 cell. The activation of STAT3 and up-regulated expression of TIMP-1 might be important molecular mechanism for promoting the viability of Burkitt and DLBCL cell.

关键词(KeyWords)： 淋巴瘤,B细胞;伯基特淋巴瘤;细胞培养;信号转导子与转录活化子-3;基质金属蛋白酶组织抑制因子-1;白细胞介素-6
lymphoma;B cell;Burkitt lymphoma;cell culture;single transducer and transcription activator-3;tissue inhibitors of metalloproteinase-1;interleukin-6

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.81160299);; 贵州省优秀人才省长基金(No.2011.125)

作者(Author): 杨文秀,李品浩,陈琴,裴媛媛
YANG Wenxiu,LI Pinhao,CHEN Qin,PEI Yuanyuan

DOI: 10.19367/j.cnki.1000-2707.2016.02.002

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