贵州医科大学学报

2017, v.42;No.207(12) 1375-1378

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电镜下两种嗜尸性蝇卵表面超微结构及不同发育时期的结构变化
Morphological Characteristics of Two Kinds of Sarcosaprophagous Flyblew and Surface Ultrastructure of Flyblew at Different Developmental Stages

王启燕;张红玲;刘玉波;戴佳琳;王杰;任峥;黄江;
WANG Qiyan;ZHANG Hongling;LIU Yubo;DAI Jialin;WANG Jie;REN Zheng;HUANG Jiang;Department of Forensic Medicine in Guizhou Medical University;

摘要(Abstract):

目的:扫描电镜观察两种嗜尸性蝇卵表面的超微结构及不同发育时期的结构变化,为法医学推断死后间隔时间(PIM)提供昆虫学依据。方法:大头金蝇、巨尾阿丽蝇成虫产卵开始记为0 h,大头金蝇卵每隔2 h收集一次,巨尾阿丽蝇卵每隔4 h收集一次,直至有幼虫孵出,用扫描电镜观察两种蝇卵形态及不同发育时期蝇卵表面超微结构的变化规律。结果:大头金蝇卵特点为中区终止包绕卵孔的方式为分叉型的,整体呈"Y"字形,中区以外绒毛膜光滑,垂柱之间时有融合,卵孔一端在0~4 h变化不明显,略向外突出,卵孔周围光滑,6 h卵孔开始凹陷、皱缩,8 h凹陷明显,9 h已发育为幼虫;巨尾阿丽蝇卵特点为在中区以外绒毛膜为菱形的网状结构,垂柱分散,端部呈球形,少数并接,卵孔一端在0~4 h变化不明显,卵孔周围不规则突起,发育8 h卵孔周围变光滑,圆形卵孔可见,发育12 h卵孔消失,所在部位开始皱缩,发育16 h卵孔周围皱缩明显,18 h已发育为幼虫。结论:观察不同发育时期大头金蝇卵与巨尾阿丽蝇卵表面超微结构,可为法医学推断PIM提供昆虫形态学依据。
Objective: To observe the morphological characteristics of two kinds of sarcosaprophagous flyblew and surface ultrastructure of flyblew at different developmental stages by scanning electron microscopy( SEM),providing an entomological basis for forensic medicine to infer postmortem interval time( PIM). Methods: Oviposition of Chrysomya megacephala and Aldrichina grahami began to take 0 h,and the eggs were collected every 2 h. Aldrichina grahami flyblow was collected every 4 h,until the larvae hatched. The changes of ultrastructures of flyblow and different developmental stages of flyblow were observed by scanning electron microscope. Results: The features of chrysomya flyblow: the way to terminate the oval holes in the middle area was a bifurcated type,and the whole was " Y". The chorion was smooth and there was a fusion between the vertical columns. At the end of the ovum,the change of 0 ~ 4 h was not obvious and slightly outgoing. The pores around the ovum were smooth; the 6 h egg hole began to sag and crinkle; depression of 8 h was obvious,and 9 h had been developed as a larva. The features of Aldrichina grahami flyblew: chorionic villus outside the middle area was reticulate structure of rhomboid,vertical column dispersion,globular end as well as minority and connection. At the end of the ovum,the change of 0 ~ 4 h was not obvious and irregular protuberances around the oval foramen. The development of 8 h oval pores was smooth and visible round oval holes. The development of 12 h oval holes disappeared and the location of the eggs began to shrink. The crinkle of the 16 h egg hole was obvious,and the 18 h had been developed into the larvae. Conclusion: To observe the different developmental stages of eggs and surface ultrastructure of chrysomya Aldrichina Ali flyblow can provide morphological basis for forensic inference PIM.

关键词(KeyWords): 嗜尸性蝇卵;形态学;超微结构;电镜;法医学
sarcosaprophagous flyblew;morphology;ultrastructures;scanning electron microscopy;forensic medicine

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金主任基金(81641082);; 贵州省科技厅计划基金[黔科合LH字(2016)7360,黔科合LH字(2014)7089];; 贵州省发改委现代法医司法鉴定工程研究中心项目(黔发改高技20161345)

作者(Author): 王启燕;张红玲;刘玉波;戴佳琳;王杰;任峥;黄江;
WANG Qiyan;ZHANG Hongling;LIU Yubo;DAI Jialin;WANG Jie;REN Zheng;HUANG Jiang;Department of Forensic Medicine in Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.12.003

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