汪闯,周建奖,谢渊,赵艳
WANG Chuang,ZHOU Jianjiang,XIE Yuan,ZHAO Yan

• 1:贵阳医学院分子生物学重点实验室
• 2:贵阳医学院

摘要(Abstract)：

目的:研究胃泌素(GAS)在多种肿瘤中的表达。方法:应用免疫组织化学检测由40例肿瘤组织组成(含87个点)的组织芯片中GAS的表达,同时检测临床相应的肿瘤组织和胃癌细胞SGC-7901、AGS、肺癌细胞A549、肝癌HepG2、乳腺癌细胞MCF-7中GAS和胃泌素受体(CCK-BR)的表达。结果:GAS主要表达于细胞质,组织芯片检测发现GAS在食管鳞状细胞癌、胃腺癌、结肠腺癌、肝细胞性肝癌、肺鳞状细胞癌、乳腺浸润性导管癌中表达;GAS在临床肿瘤标本胃癌、肝癌、肺癌、乳腺癌和胃癌细胞SGC-7901、AGS、肺癌细胞A549、肝癌细胞HepG2及乳腺癌细胞MCF-7中证实GAS阳性表达,同时发现CCK-BR也在胃癌、肝癌、肺癌和乳腺癌组织及SGC-7901、AGS、A549、HepG2、MCF-7细胞株中表达。结论:GAS和CCK-BR在消化道和非消化道肿瘤中表达,胃泌素/CCK-B受体环可能在胃癌、肺癌、肝癌及乳腺癌中起重要作用。
Objective: To study the expression of gastrin in various tumors. Methods: Immunohistochemical method was adopted. The expression of gastrin was detected in tissue chips of 40 cases of tumor tissues( 87 points included). As well,gastrin and gastrin receptor( CCK-BR) expression in clinical corresponding tumor tissues and gastric cancer cells SGC-7901,and AGS,lung cancer cells A549,liver cancer cells HepG2,breast cancer cells MCF-7 were detected. Results: Gastrin expression was mainly found in cytoplasm. Tissue chip results showed that gastrin expression was found in esophageal squamous cell carcinoma,gastric adenocarcinoma,colon adenocarcinoma,hepatocellular carcinoma,lung squamous cell carcinoma and breast invasive ductal carcinoma. The positive expression of gastrin was proved in clinical specimens of gastric cancer,liver cancer,lung cancer,and breast cancer,and in gastric cancer cells SGC-7901,and AGS,lung cancer cell A549,liver cancer cell HepG2,and breast cancer cell MCF-7. CCK-BR expression was further confirmed in the tissues of gastric cancer,liver cancer,lung cancer,breast cancer and cell strains of SGC-7901,AGS,A549, HepG2,and MCF-7. Conclusion: Gastrin expression occurs in digestive tract and non-digestive tract tumors,and the autocrine loop constructed by gastrin and CCK-BR have important function in gastric cancer,lung cancer,liver cancer and breast cancer.

关键词(KeyWords)： 胃泌素;肿瘤;受体,胃泌素;免疫组织化学;组织微阵列/组织芯片技术
gastrin;tumor;receptors,gastrin;immunohistochemical method;tissue microarray/ tissue chip

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81260303);; 贵州省科技攻关计划项目(黔科合SY字[2011]3067号);; 贵州省科学技术基金项目[黔科合J字(2011)2039号]

作者(Author): 汪闯,周建奖,谢渊,赵艳
WANG Chuang,ZHOU Jianjiang,XIE Yuan,ZHAO Yan

DOI: 10.19367/j.cnki.1000-2707.2014.02.002

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