贵州医科大学学报

2017, v.42;No.196(01) 45-51

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叶酸偶联荧光量子点脂质体纳米探针的合成
Synthesis of Folate-conjugated Quantum Dots Liposome Nanoprobes

胡珺;杨畅;陈思颖;赵青;王永林;
HU Jun;YANG Chang;CHEN Siying;ZHAO Qing;WANG Yonglin;School of Pharmacy,Guizhou Medical University;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM ( Ministry of Education),Guizhou Medical University;

摘要(Abstract):

目的:制备荧光量子点和叶酸偶联荧光量子点脂质体纳米探针,初步确证探针的性质,为相关肿瘤的早期荧光诊断奠定基础。方法:采用水相合成法合成3-巯基丙酸修饰的水溶性CdTe量子点,将脂质材料与叶酸分子连接,通过薄膜水化法制备得到叶酸偶联的荧光量子点脂质体纳米探针,利用紫外可见吸收光谱(UVvis)、红外光谱(IR)、分子荧光光谱法(MFS)、核磁共振(NMR)等方法确证荧光量子点、叶酸偶联脂质材料、叶酸偶联荧光量子点脂质体纳米探针性质;于-4℃冰箱内贮存6个月,观察叶酸偶联荧光量子点脂质体纳米探针的稳定性。结果:荧光量子点的荧光性质可随着反应时间的变化而变化,红外光谱、紫外可见吸收光谱、核磁共振氢谱证实叶酸偶联脂质材料合成成功;合成得到的叶酸偶联荧光量子点脂质体纳米探针平均粒径为462.9 nm,zeta电位为-0.072 2 mV,在520 nm处有最大荧光发射峰;放置6个月后,荧光性质未见明显变化。结论:叶酸偶联脂质体成功将CdTe量子点包裹,制备得到荧光强度高、性质稳定的纳米探针。
Objective: To prepare fluorescence quantum dots and folate-conjugated quantum dots liposome nanoprobes for the molecular fluorescence diagnosis of early tumors,and to characterize their properties preliminarily. Methods: Water-soluble Cd Te quantum dots modified with 3-mercaptopropionic acid was synthesized in water. Lipid material was linked with folic acid,and folate-conjugated fluorescent quantum dots liposome nanoprobes were prepared by thin membrane hydration method. UV-vis spectra,IR spectra,MFS and NMR detection methods were used to characterize the quantum dots,folate-conjugated lipid materials,and folate-conjugated quantum dots liposomes nanoprobe. The stability of folate-conjugated quantum dots liposomes nanoprobe was observed after stored in-4 ℃ for 6months. Results: The Fluorescence properties of QDs changed with the reaction time. The success of synthesis of folate-conjugated liposomes was proved by UV-vis spectra,IR spectra,MFS and NMR detection methods. The average diameter of folate-conjugated quantum dots liposome nanoprobes was462. 9 nm,and zeta potential was-0. 072 2. The maximum fluorescence emission peak was located at520 nm,and the fluorescence did not change after 6 month storage. Conclusion: Quantum dots were successfully entrapped in the folate-conjugated liposomes,and nanoprobes with strong and stable fluorescent have been successfully prepared.

关键词(KeyWords): 叶酸;偶联;荧光量子点;脂质体;纳米探针
folic acid;conjugation;fluorescence quantum dots;liposomes;nanoprobes

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金项目[黔科合J字(2014)2019号];; 贵州医科大学博士启动基金(院基金合同字第014号);; 贵州省卫生计生委科学技术基金项目(gzwjkj2015-1-027);; 贵州省科技厅-贵州医科大学科学技术联合基金项目[黔科合LH字(2016)7364号];; 贵州省教育厅现代药物研究开发协同创新中心[黔教合协同创新字(2013)04号]

作者(Author): 胡珺;杨畅;陈思颖;赵青;王永林;
HU Jun;YANG Chang;CHEN Siying;ZHAO Qing;WANG Yonglin;School of Pharmacy,Guizhou Medical University;Guizhou Provincial Key Laboratory of Pharmaceutics,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM ( Ministry of Education),Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.01.010

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