蒋天盛,李春来,袁洪涛
JIANG Tiansheng,LI Chunlai,YUAN Hongtao

• 1:贵阳市第一人民医院

摘要(Abstract)：

目的:观察气腹预处理后大鼠肝组织中磷脂酰肌醇-3激酶/蛋白激酶B(PI3K/Akt)信号通路的变化。方法:健康SD大鼠30只,随机均分为假气腹组、气腹组和气腹预处理组;气腹组大鼠脐旁插入气腹针并连接SN/REF气腹机充入二氧化碳(CO_2)建立气腹模型(腹压保持在15 mm Hg,持续90 min后解除气腹),气腹预处理组在建立气腹前先进行预处理(充气5 min,放气5 min,重复2次),其余同气腹组;假气腹组大鼠脐旁插入气腹针但不充入CO_2气体;实验结束后处死大鼠;取同一部位肝组织,采用Western Blot检测肝脏中Akt、p-Akt蛋白的表达,采用TUNEL法检测肝细胞凋亡。结果:与假气腹组比较,气腹组大鼠肝脏中Akt及p-Akt的表达显著减少,而肝细胞凋亡率则显著增加,差异具有统计学意义(P<0.01);与气腹组比较,气腹预处理组大鼠肝脏中Akt及p-Akt的表达显著增高,而肝细胞凋亡率则明显较少,差异具有统计学意义(P<0.05)。结论:PI3K/Akt信号通路可能参与了气腹预处理对大鼠肝组织的保护作用。
Objective: To observe the change of PI3K/Akt signaling pathway in liver tissue of pneumoperitoneum preconditioning rats. Methods: 30 healthy SD rats were divided into sham pneumoperitoneum group,pneumoperitoneum group and pneumoperitoneum preconditioning group randomly,10 rats in each group. Inserting the veress beside the navel of rats and connecting SN / REF pneumoperitoneum machine to insufflate CO_2 for 90 minutes to establish the pneumoperitoneum model,abdominal pressure maintained at 15 mm Hg. The pneumoperitoneum preconditioning group was subjected to 5min of insufflation and 5 min of deflation,repeat for 2 times,then following the same practice as pneumoperitoneum group. The rats of sham pneumoperitoneum group were inserted needles but not filling CO_2. Changes of PI3 K / Akt signaling pathway were detected by western blot. The apoptosis of hepatocytes was detected by TUNEL. Results: Comparing with sham pneumoperitoneum group,the expression of total Akt and p-Akt of pneumoperitoneum group were significantly decreased( P < 0. 01),while the apoptosis of hepatocytes was increased; comparing with pneumoperitoneum group,the expression of total Akt and p-Akt of pneumoperitoneum preconditioning group were significantly increased( P <0. 05),while the apoptosis of hepatocytes was decreased. Conclusion: PI3 K / Akt signaling pathway may be involved in the protective effects of pneumoperitoneum preconditioning on liver tissue of rats.

关键词(KeyWords)： 模型,动物;气腹,人工;预处理;蛋白激酶B;大鼠,Sprague-Dawley
model,animal;pneumoperitoneum,artificial;preconditioning;protein kinase B;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵阳市卫生局科学技术计划项目[(2014)筑卫科技合同字第2号]

作者(Author): 蒋天盛,李春来,袁洪涛
JIANG Tiansheng,LI Chunlai,YUAN Hongtao

DOI: 10.19367/j.cnki.1000-2707.2016.05.017

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