张婷,单可人,何燕,徐仕清,吴昌学,周曙光,李毅,王婵娟,赵艳,官志忠
ZHANG Ting1,SHAN Keren1,HE Yan1,XU Shiqing2,WU Changxue1,ZHOU Shuguang3,LI Yi1,WANG Chanjuan1,ZHAO Yan1,GUAN Zhizhong1(1.The Key Lab of Molecular Biology

• 1:贵阳医学院分子生物学重点实验室
• 2:毕节市疾病预防控制中心
• 3:长顺县疾病预防控制中心

摘要(Abstract)：

目的:探讨燃煤型氟中毒地区人群过氧化氢酶(CAT)、谷胱甘肽S-转移酶(GSTs)活力与氧化应激的关系。方法:于贵州省毕节市鸭池镇燃煤型氟中毒非改灶村抽取245名村民作为非干预组,于该市长春镇燃煤型氟中毒改灶村抽取240名村民作为干预组,于非地方性氟中毒长顺县白云山镇抽取247名村民作对照组,利用比色法分别测定CAT、GSTs的活力。结果:非干预组血浆CAT活力和GSTs活力低于干预组和对照组,干预组低于对照组,各组间差异有统计学意义(P<0.05);各组间CAT、GSTs活力无性别差异(P>0.05)。结论:过量氟可能使体内自由基代谢失衡,推测通过人为干预氟的摄入,有助于机体恢复氧化平衡。
Objective: To investigate the relationship of plasma catalase(CAT) and glutathione S-transferase(GSTs) activity levels with oxidation stress in patients with coal-burning caused fluorosis.Methods: 245 villagers that use unchanged stoves in coal-burning fluorosis area were selected as non-intervention group(group N),240 villagers using improved stoves in fluorosis endemic area were chosen as intervention group(group I),and 247 villagers in non-fluorosis-endemic area were as control group(group C).The activities of CAT and GSTs of them were tested with Spectrophotometer.Results: The activities of plasma CAT and GSTs of group N were significantly lower than those of group I(P<0.05),and the latter ones were significantly lower than those of group C(P<0.05).No significant difference of the two enzymes based on sex was found in all the 3 groups.Conclusion: Excessive intaking of fluorin might cause unbalance of free radical metabolism,and artificial intervention of fluorin intaking could help to recover the balance of oxidation.

关键词(KeyWords)： 氟化物中毒;过氧化氢酶;谷胱甘肽转移酶;氧化性应激
fluoride poisoning;catalase;glutathione transferase;oxidative stress

Abstract:

Keywords:

基金项目(Foundation): 国家科技部国际合作项目(2010DFB30530);; 国家自然科学基金资助项目(30760224);; 贵州省长专项资金资助项目(黔省专合字200980)

作者(Author): 张婷,单可人,何燕,徐仕清,吴昌学,周曙光,李毅,王婵娟,赵艳,官志忠
ZHANG Ting1,SHAN Keren1,HE Yan1,XU Shiqing2,WU Changxue1,ZHOU Shuguang3,LI Yi1,WANG Chanjuan1,ZHAO Yan1,GUAN Zhizhong1(1.The Key Lab of Molecular Biology

DOI: 10.19367/j.cnki.1000-2707.2010.04.002

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