高秀丽,宋智魁,王清忱,孟小夏,舒阳,张敏,王鹏娇
GAO Xiuli1,SONG Zhikui1,WANG Qingchen2,MENG Xiaoxia1,SHU Yang1,ZHANG Min1,WANG Pengjiao1(1.School of Pharmacology

• 1:贵阳医学院药学院
• 2:贵州省人民医院

摘要(Abstract)：

目的:建立一种能够稳定检测贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法。方法:采用Tricine-SDS-PAGE电泳方法,分离胶浓度为12%,交联度为3%,以blue-silver染色法进行染色,对贵州豆豉激酶组分进行分析。结果:贵州豆豉激酶组分经Tricine-SDS-PAGE电泳后,在蛋白Marker在20.1～43 kDa之间,出现5条较清晰的条带。结论:Tricine-SDS-PAGE电泳方法稳定可靠,能够用于贵州豆豉激酶组分的检测。
Objective: To establish a tricine-SDS-PAGE method for stable determination of kinase components in Guizhou douchi.Method: Tricine-SDS-PAGE was used,of which the gel concentration and cross-linking density were 12% and 3%,respectively.After electrophoresis,the gel was stained with "blue-silver" method.Results: Five bands between 20.14 kd and 43 kd were found on the electrophoretogram.Conclusion: Tricine-SDS-PAGE could detect the components of Guizhou douchi steadily and reliably.

关键词(KeyWords)： 电泳;贵州豆豉激酶组分;纤溶酶;发酵
electroproresis;kinase components in Guizhou douchi;plasmin;fermentation

Abstract:

Keywords:

基金项目(Foundation): 贵阳市科技局[(2009)筑科农合同字第2-009号]

作者(Author): 高秀丽,宋智魁,王清忱,孟小夏,舒阳,张敏,王鹏娇
GAO Xiuli1,SONG Zhikui1,WANG Qingchen2,MENG Xiaoxia1,SHU Yang1,ZHANG Min1,WANG Pengjiao1(1.School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2010.06.010

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