孙江龄,何常,刘俊玲,程明亮
SUN Jiangling,HE Chang,LIU Junling,CHENG Mingliang

• 1:贵阳医学院病理学教研室
• 2:贵阳医学院附院病理科
• 3:贵阳医学院附院感染科

摘要(Abstract)：

目的:研究不同白酒对酒精性肝纤维化、肝硬化合并肝癌大鼠肝组织中Kruppule样因子6(Klf 6)及相关基因表达的影响。方法:运用2种白酒和黄曲霉素B1(AFB1)对大鼠进行处理,制备酒精性肝纤维化、肝硬化合并肝癌大鼠模型。用免疫组织化学法检测模型大鼠肝脏组织Klf 6、转化生长因子-β1(TGF-β1)、p21及α-平滑肌动蛋白(α-SMA)的表达水平。RT-PCR技术检测大鼠肝脏组织中Klf 6基因mRNA表达水平。结果:成功制备了酒精性肝纤维化、肝硬化合并肝癌大鼠模型,不同白酒诱导大鼠肝纤维化、肝硬化合并肝癌的程度不同。Klf 6、p21在白酒A组(AD组和AG组)的表达率高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05);TGF-β1,α-SMA在白酒B组(BD组和BG组)表达高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05)。RT-PCR结果显示,白酒A组(AD组和AG组)肝组织Klf 6基因的mRNA表达高于白酒B组(BD组和BG组),差异有统计学意义(P<0.05)。结论:不同白酒对酒精性肝纤维化,肝硬化合并肝癌大鼠肝脏Klf 6、p21、TGF-β1和α-SMA的表达不同,可能是导致酒精性肝病(ALD)的程度不同的机制之一。
Objective: To discuss the expression of Klf 6 and related gene in hepatic fibrosis,and hepatocirrhosis with hepatoma tissue rats administered with different liquor. Methods: The hepatic fibrosis and hepatocirrhosis with hepatoma rat model was made with aflatoxin B1( AFB1) and 2 liquor( liquor A and B with low and high content) respectively,and the control rats were administered with distill water. The protein expressions of Kruppel-like factor 6( Klf 6),transforming growth factor-β1( TGF-β1),p21,and alpha smooth muscle actin( α-SMA) in liver were detected with immunohistochemistry method,and the mRNA level were evaluated with RT-PCR after the rats were decapitated.Results: The hepatic fibrosis and hepatocirrhosis with hepatoma rat model was made successfully,and rat models induced by different liquor showed pathological changes with different severity. The expression of Klf 6( protein and mRNA level) and p21( protein level) of liquor A low-content group( group AD) and high-content group( group AG) were higher than those of liquor B low-content group( group BD) and high-content group( group BG) with statistical significance( P < 0. 05). However,the protein expression of TGF-β1 and α-SMA increased in BD and BG groups contrasted with AD,AG and control groups( P < 0. 05). Conclusions: The degree of the effects of different liquor on the expression of Klf 6,TGF-β1,p21,and α-SMA is different,which may contribute to the different inducing effects on hepatic fibrosis,hepatocirrhosis and hepatocirrhosis with hepatoma.

关键词(KeyWords)： 肝疾病,酒精性;纤维化;肝硬化,酒精性;肝肿瘤;Kruppule样因子6;蛋白质,p21;α-平滑肌动蛋白
liver diseases,alcoholic;fibrosis;liver cirrhosis,alcoholic;liver neoplasms;Kruppulelike factor 6;protein p21;α-smooth muscle actin

Abstract:

Keywords:

基金项目(Foundation): 国家重点研究计划(973)课题(NO.2011CB512114);; 贵州省科技计划课题(黔科合SZ[2011]3001)

作者(Author): 孙江龄,何常,刘俊玲,程明亮
SUN Jiangling,HE Chang,LIU Junling,CHENG Mingliang

DOI: 10.19367/j.cnki.1000-2707.2013.06.008

参考文献(References)：

• [1]中华医学会肝病学分会脂肪肝和酒精性肝病学组.酒精性肝病诊疗指南(2010年修订版)[S].中华肝脏病杂志,2010(3):167-170.
• [2]程明亮,吴君,张文胜,等.茅台酒对肝脏的作用及其影响的实验研究[J].中华医学杂志,2003(83):237-240.
• [3]吴君,程明亮,王海琴,等.茅台酒与肝病关系的流行病学调查及病理组织学研究[J].世界华人消化杂志,2001(12):1374-1378.
• [4]Narla G,Heart KE,Reeves HL.Klf 6,a candidate tumor suppressor gene mumted in prostate cancer[J].Science,2001(5551):2563-2566.
• [5]王少平,亢黎莉.Klf 6基因在原发性肝癌中的表达[J].华中科技大学学报,2008(3):343-346.
• [6]李向荣,林星余,肖忠秀,等.p21结构功能与研究新进展[J].医学综述,2008(14):2094-2097.
• [7]何常,邹美平,张人华,等.Klf 6、p21WAF1/CIP1、CyclinD1在结直肠癌中的表达及意义[J].山东医药,2011(16):28-29.
• [8]杨平,曹杰,梁立源,等.Klf 6蛋白在散发性结直肠癌组织中的表达及其与p21蛋白的关系[J].中华实验外科杂志,2010(10):1480-1482.
• [9]Choi SS,Diehl AM.Epithelial-to-mesenchymal transitions in the liver[J].Hepatology,2009(50):2007-2013.
• [10]Kaimo A,Potter J,Kaimori JY,et a1.Transforming growth factor betal induces an epithelial-to-mesenchymal transition state in mouse hepatocytes in vitro[J].J Biol chem,2007(30):22089-22101.

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